African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Effect of iron limitation on the production of Streptolysin S (SLS) of Streptococcus pyogenes Group A

  Badaruddin A. Memon1* and T. H. Birkbeck2
  1Department of Microbiology, Shah Abdul Latif University Khairpur, Sindh, Pakistan. 2Institute of Biomedical Sciences, Division of Infection and Immunity, Black Joseph Building, University of Glasgow, G12, 8QQ, Glasgow, UK.
Email: [email protected]

  •  Accepted: 31 October 2011
  •  Published: 16 November 2011



The effect of iron on the growth of Streptococcus pyogenes Group A and its influence on Streptolysisn S (SLS) production was investigated. Two strains of S. pyogenes used in this study C203S and 55903 M were grown in iron-restricted medium. The iron chelator, (Ethylenediamindi-(o- hydroxyphenyl-acetic acid) (EDDA) was added at a concentration giving a 10-fold molar excess over Fe. Successful growth of strains of S. pyogenes was obtained suggesting no absolute requirement for iron in the growth and the yield of SLS was not affected by the concentration of iron in the growth medium.


Key words: Streptolysin S, Streptococcus pyogenes Group A, iron requirement.