African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Erythritol biosynthesis by Yarrowia lipolytica yeast under various culture conditions

  Anita RywiÅ„ska*, Ludwika Tomaszewska and Waldemar Rymowicz
Department of Biotechnology and Food Microbiology Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wrocław, Poland.
Email: [email protected]

  •  Accepted: 12 June 2013
  •  Published: 05 July 2013



Erythritol is a natural compound of great interest in food production because of its very low caloric value, lack of off-taste and lack of side-effects from the gastric system even when consumed with in excess. In the present study, the effect of agitation rates from 500 to 1100 rev/min on biomass and erythritol production on glycerol media by an acetate-negative mutant of Yarrowia lipolytica Wratislavia K1 in batch culture was studied. At a constant aeration rate of 0.36 vvm, the agitation rate between 500 and 900 rev/min wasfound suitable for efficient erythritol production. In the pure glycerol-containing media the erythritol production of 40.7 g/l, corresponding to a 0.28 g/g yield, was achieved at 800 rev/min. The application of crude glycerol at 800 rev/min and 0.6 vvm elicited an increase in erythritol concentration to 58.2 g/l corresponding to a 0.38 g/g yield and a productivity of 0.78 g/lh.


Key words: Yarrowia lipolytica, erythritol, glycerol, agitation rate, aeration rate.