African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Antimicrobial susceptibility profile of Enterococcus species isolated from cows with clinical mastitis and from bulk milk tanks in Brazil

de Moraes Gustavo Nunes
  • de Moraes Gustavo Nunes
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Guimarães Felipe de Freitas
  • Guimarães Felipe de Freitas
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Fornazari Felipe
  • Fornazari Felipe
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Joaquim Sâmea Fernandes
  • Joaquim Sâmea Fernandes
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Guerra Simony Trevizan
  • Guerra Simony Trevizan
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
de França Danilo Alves
  • de França Danilo Alves
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Possebon Fábio Sossai
  • Possebon Fábio Sossai
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Pantoja Jose Carlos de Figueiredo
  • Pantoja Jose Carlos de Figueiredo
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Lucheis Simone Baldini
  • Lucheis Simone Baldini
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Rall Vera Lucia Mores
  • Rall Vera Lucia Mores
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Hernandes Rodrigo Tavanelli
  • Hernandes Rodrigo Tavanelli
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Ribeiro Marcio Garcia
  • Ribeiro Marcio Garcia
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar
Leite Domingos da Silva
  • Leite Domingos da Silva
  • Department of Genetics, Evolution, Microbiology and Immunology Institute of Biology. Campinas, Brazil.
  • Google Scholar
Langoni Helio
  • Langoni Helio
  • Department of Veterinary Hygiene and Public Health. College of Veterinary Medicine and Zootechnics. São Paulo State University "Júlio de Mesquita Filho", Botucatu, São Paulo, Brazil.
  • Google Scholar


  •  Received: 01 August 2022
  •  Accepted: 28 September 2022
  •  Published: 28 February 2023

Abstract

Vancomycin-resistant enterococci (VRE) have been reported as a major hazard to human and animal health, causing problems like clinical mastitis in dairy cattle and nosocomial infections in humans. The aim of this study was to determine the in vitro microbial susceptibility profile of enterococci isolated from dairy farms in São Paulo and Minas Gerais states, Brazil. A total of 310 samples of Enterococcus species from bovine mastitis (52) and bulk tanks (258) were analyzed. The sensibility profile was studied by the disk diffusion method, and the isolates intermediately resistant to vancomycin were submitted to the minimum inhibitory concentration (MIC) test. The enterococci also were classified according to their multiresistance profile. All isolates were sensitive to vancomycin. Most of the isolates from the bulk tanks were resistant to cephalexin (93.8%), novobiocin (98.8%), cefoxitin (91.9%) and oxacillin (91.9%), while those isolated from mastitis presented a high resistance to trimethoprim/sulfamethoxazole (84.7%), novobiocin (100%), cefoxitin (88.5%) and oxacillin (80.8%). All isolates were sensitive to vancomycin (VSE). The high prevalence of isolates resistant to multiple antimicrobials emphasizes the risks existing in the use of ineffective antibiotics.

 

Key words: Enterococci, mastitis, multiresistence, milk, vancomycin.