African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Short Communication

In vitro antimicrobial activity of leaf extract of Berlina grandiflora Hutch. and Dalz.

  Akuodor G. C.1*, Onyewenjo S. C.2, Anyalewechi N. A.1, Essien A. D.3 Akpan J. L.4, Okoroafor D. O.5 and Okere M. O.6
  1Department of Pharmacology and Toxicology National Institute for Pharmaceutical Research and Development (NIPRD) P. M. B. 21 Garki, Abuja, Nigeria. 2Departments of Biology/ Microbiology, Federal Polytechnic, Owerri, Nigeria. 3Department of Pharmacology, Faculty of Basic Medical Sciences, University of Calabar, Nigeria. 4Departments of Pharmacology, Faculty of Clinical Medicine, Ebonyi State University, Abakiliki, Nigeria. 5Departments of Pharmacognosy and Phytotherapy, Faculty of Pharmacy, University of Port Harcourt, Nigeria. 6Laboratory Unit, Kings Care Hospital Abuja, Nigeria.
Email: [email protected]

  •  Accepted: 19 April 2011
  •  Published: 04 June 2011

Abstract

 

Antimicrobial properties of Berlina grandiflora Hutch. and Dalz. leaf extract were investigated against both clinical and laboratory isolates. Susceptibility of these isolates to the extract was determined using disc diffusion method. The antimicrobial screening had wide range of activity on Escherichia coli, Stapylococcus aureus and Streptococcus spp. The diameter of zone of inhibition by the extract was 7, 8.7 and 9 mm respectively. The minimum inhibitory concentrations (MIC) were 32.81, 19.38 and 11.72 µg/ml for E. coli, S. aureus and Streptococcus spp. Based on the current findings, it can be concluded that B. grandiflora has antimicrobial activity against certain microorganisms.

 

Key words: Berlina grandiflora, antimicrobial agent, minimum inhibition concentration, in vitro.