African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Influence of bispyribac sodium on nitrogenase activity and growth of cyanobacteria isolated from paddy fields

Gulten Okmen* and Aysel Ugur
Department of Biology, Faculty of Science, Mugla University, Mugla 48127, Turkey.
Email: [email protected]

  •  Accepted: 30 July 2011
  •  Published: 16 September 2011


The goal of this study was to determine the effect of bispyribac-sodium on the nitrogenase activities and growth of cyanobacteria isolated from paddy fields. Ten cyanobacterialspecies were used in this study. Cyanobacterial species were isolated from soil and water samples obtained from rice fields in Corum, Turkey. Among all Anabaena strains, the maximum activity was determined in Anabaena sp. O-22 (2.26 µl ethylene/mg.h) whereas; the lowest activity was shown in Gloeothece sp. O-Y (0.04 µl ethylene/mg.h).The maximum inhibition was seen in Anabaena sp. O-22, Synechocystis sp. O-X andAnabaena sp. O-16 in 100 µg/ml bispyribac-sodium concentration. Although low bispyribac-sodium concentrations somewhat stimulated growths of Anabaena sp. O-X2, O-Ç, O-4, O-16, O-8 and Synechocystis sp. O-X, the biomass of all cultures were severely inhibited at higher concentrations. The growths of Anabaena sp. O-22 andSynechocystis sp. O-X completely repressed at 100 µg/mL and at higher bispyribac-sodium concentrations, whereas, Anabaena O-X2, O-6, O-4 and O-16 completely suppressed at 500 µg/mL bispyribac-sodium concentration. The end of the studyAnabaena sp. O-22 has been proposed as biofertilizer. The results obtained may be useful for the production of rice.


Key words: Cyanobacteria, nitrogenase activity, herbicide.