African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry

Akosua B. Karikari
  • Akosua B. Karikari
  • Department of Clinical Microbiology, University for Development Studies Tamale, Ghana.
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Kwasi Obiri-Danso
  • Kwasi Obiri-Danso
  • Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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Enoch H. Frimpong
  • Enoch H. Frimpong
  • Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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Karen A. Krogfelt
  • Karen A. Krogfelt
  • Statens Serum Institute, Microbiology and Infection Control, Denmark.
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  •  Received: 05 October 2016
  •  Accepted: 04 November 2016
  •  Published: 21 February 2017

Abstract

Poultry meat and products are major transmission routes of human campylobacteriosis. The aim of this study was to determine the numbers and antibiogram profile of Campylobacter isolates from slaughtered broiler and layer birds. One hundred and sixty caecal and one hundred and thirty two carcasses were randomly sampled at the Kejetia poultry slaughter, isolated on charcoal-cefoperazone-deoxycholate agar (CCDA) and confirmed by API CAMPY and their resistance profiles assessed by Kirby-Bauer disk diffusion. Prevalence was 22.5 and 21.9% in the faecal and carcasses, respectively with no significant differences. Species identified among faecal isolates were Campylobacter jejuni (42%), Campylobacter coli (28%), Campylobacter lari (22%) and Campylobacter hyo-intestinalis (8%) while 79% C. jejuni, 14% C. coli, 4% C. jejuni sub sp. doylei, and 3% C. lari were obtained from the carcasses. Resistance to the β-lactams ranged from 75 to 100%, 41 to 86% to the quinolones, 14 to 36% to the aminoglycosides, 100% to erythromycin, 97 to 100% to tetracycline, 72 to 83% to chloramphenicol and 90 to 94% to trimethoprim sulfamethoxazole. All species were sensitive to imipenem, but 100% of isolates were multidrug resistant. Contamination of carcasses with multidrug resistant strains of Campylobacter is a threat to handlers and consumers and of major public health issue.

Key words:  Multidrug resistance, faeces, carcass, poultry, Kumasi, Ghana.