African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5227

Full Length Research Paper

Bioremediation of 3,5-dinitrobenzoic acid and aniline by a Corynebacterium sp.

  Ahmed, Y. M.1,3*, Kumosani, T. A.1 and Sabir, J. S. M.2
  1Biochemstry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia 2Biolgy Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia 3National Research Center, Microbial Biotechnology Department, Egypt.
Email: [email protected]

  •  Accepted: 25 June 2013
  •  Published: 13 September 2013

Abstract

 

Corynebacterium sp was isolated from the soil by using 3-5 dinitro benzoic acid (DNB) as a sole carbon source. The highest rate of degradation of aniline (AN) or DNB was found in the exponential phase of the growth of the bacterium. After 24 h, about 50% of DNB and 30% of AN were degraded by Corynebacterium spAt a concentration of 0.5 to 1 g/L of AN or DNB, good growth was obtained and the protocatechuic acid was detected. The optimum concentration of yeast extract was 2 g/l. Catechol 1-2 dioxygenase was induced in the cells grown on a medium containing AN or DNB. A significant activity of this enzyme was detected, which means that ortho cleavage pathway may be present inCorynebacterium sp.

 

Key words: Corynebacterium sp., degradation, dinitrobenzoic acid (DNB), aniline (AN),protocatechuic acid, catechol, catechol 1-2 dioxygenase, Ortho cleavage, high performance liquid chromatography (HPLC).