African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Microbiological assay of the active component of ampicillin in ampicillin and ampicillin/cloxacillin suspensions using Bacillus megatharium NCTC 10342A76 as indicator organism

Udobi Chinweizu Ejikeme* and Onaolapo Josiah Ademola
  College of Science and Technology, Kaduna Polytechnic, Kaduna, Nigeria. Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria, Nigeria.
Email: [email protected]

  •  Accepted: 19 August 2009
  •  Published: 04 January 2010

Abstract

 

Fifty one different samples from 5 different manufacturers of ampicillin and ampicillin/cloxacillin suspensions were tested for their levels of active components of ampicillin using the microbiological assay method. Bacillus megathariumNCTC10342A76 was selected from amongst a wide range of organisms screened for this purpose because of its detected suitability. Thin layer chromatography and infra red spectrophotometric analysis confirmed the presence of ampicillin in all the samples and the results obtained showed that the percentage ampicillin content ranged from 57 - 162. Products of MAN-A07 had ampicillin levels less than that recommended by the reference books while one batch from MAN A06 had ampicillin levels higher than the recommended levels. This work is discussed in the context of the proliferation and misuse of ampicillin especially for children.

 

Key words: Microbiological assay, Bacillus megatharium, ampicillin, cloxacillin, chromatography.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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