African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Infrared spectroscopy: Methods for investigating cellular components of phytopathogenic fungi response to temperature stress

  Mathukorn Sompong1, Kanjana Thumanu2, Inchana Prakhongka1, Bhop Burapatpong1, Dusit Athinuwat3, Sutruedee Prathuangwong4 and Natthiya Buensanteai1*
  1School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000 Thailand. 2Synchrotron Light Research Institute (public organization), Ministry of Science and Technology of the Royal Thai Government, Muang, 30000 Nakhonratchasima, Thailand. 3Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900 Thailand. 4Major of Organic Farming Management, Faculty of Science and Technology, Thammasat University, 12121, Pathumthani, Thailand.
Email: [email protected]

  •  Accepted: 21 May 2013
  •  Published: 23 August 2013

Abstract

 

Fourier-transform infrared (FTIR) spectroscopy is sensitive method for the investigation of fungi biochemical composition changes in cells. FTIR spectroscopy can be used to study fungal cell biology by fingerprinting varieties of carbohydrates, proteins, and lipids at about 6 µm spatial resolution. FTIR can distinguish fungal changes during stressed conditions, exhibit dramatic biochemical changes without obvious morphological effects.The objective of this study was to test the hypothesis that the temperature stress, as one of the most important environmental conditions, plays a critical influence on fungal cellular components involved in pathogenic mechanisms. In the current study, we investigated the impact of temperature on the changes of cellular components of phytopathogenic fungi,Sclerotium rolfsii and Colletotrichum gloeosporioides using FTIR spectroscopy with respect to the lipid, protein and polysaccharide composition. Our results demonstrate that fungal growth of S. rolfsii and C. gloeosporioides obviously increased at temperature of32°C. The culture of S. rolfsii and C. gloeosporioides grown at 32°C shows higher content of the lipid as shown in the spectral regions of CH stretching and bending bands when compared with those of the fungi culture under temperature at 28°C, indicating that all these indicators played role in temperature stress condition in S. rolfsii and C. gloeosporioides, probably due to enhanced activity of the fungal metabolism pathway and cell wall protection. Our findings illustrate that temperature stress caused increase biomass and the lipid composition that might be these key biochemical composition help phytopathogenic fungi S. rolfsii and C. gloeosporioides cope for survival at higher temperature. FTIR spectroscopy can be used as a new and sensitive tool to examine the biochemical changes within the fungal cell. This technique allows us to reveal structural chemical makeup and features within the different fungal growing conditions.

 

Key words: Sclerotium rolfsiiColletotrichum gloeosporioides, temperature response,cellular composition, FTIR spectroscopy.