African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5238

Full Length Research Paper

RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Côte d’Ivoire

Audrey A. Addablah
  • Audrey A. Addablah
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar
Solange Kakou-Ngazoa
  • Solange Kakou-Ngazoa
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar
Eric E. Akpa
  • Eric E. Akpa
  • Laboratoire de Pharmacodynamie Biochimique, Université Félix Houphouët-Boigny, 01 BP V34 Abidjan 01, Côte d’Ivoire.
  • Search for this author on:
  • Google Scholar
Emmanuella Adioumani
  • Emmanuella Adioumani
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar
Fred M’Bourou Ndombi
  • Fred M’Bourou Ndombi
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar
Serge Aoussi
  • Serge Aoussi
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar
Mireille Dosso
  • Mireille Dosso
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Search for this author on:
  • Google Scholar


  •  Received: 01 June 2021
  •  Accepted: 14 September 2021
  •  Published: 31 October 2021

Abstract

The application of bacteriophages biocontrol requires the formulation of genetically distinct bacteriophages in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered a cheap, reproducible, and readily applicable tool in detecting phage diversity compared to other molecular techniques such as whole-genome sequencing. We used in this study the RAPD-PCR technique to assess the genetic diversity of 28 bacteriophages infecting Pseudomonas aeruginosa and Staphylococcus aureus. According to their RAPD profiles, isolated phages were grouped into 2 main clusters which included phages from the same host. The typing by RAPD-PCR of newly isolated phages was useful to assess the genetic diversity bypassing previous whole-genome sequencing analysis. These genetically distinct phages lytic against P. aeruginosa and S. aureus could potentially be used in a phage cocktail for biocontrol against these clinically and industrially relevant bacteria.

 

Key words: Phages, genetic diversity, RAPD PCR, P. aeruginosa, S. aureus.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 15 No. 10
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES