Background: Hepatitis means inflammation of liver, caused by several viruses and agents. Hepatitis B virus is one of many types of viruses can infect liver and causes inflammation and in sometimes reaching to cirrhosis and hepatocellular carcinoma. Objectives: the aim of this study is to detect the genotype of hepatitis B virus from patients in Mosul. Methods: We evaluate 25 patients presumably with HBV in acute and chronic cases whom have HBsAg positive. Viral DNA was extracted from patients serum. The genotypes was detected using Real time PCR. Results: The PCR based methodology was standardized illustrated that 21 of samples perceived genotype D while the other 4 samples conferred negative result for genotypes B, C, or D. Conclusion: The standardized Real time PCR assay is a rapid and accurate method for detection and differentiation of HBV genotypes that are more frequent in meditetrenian East and Asia, B, C and D. This method can be applied in the clinical practice.

Keywords: HBV, HBsAg, CHB