African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Article in Press

In Vitro Detection of Antibacterial Activity of Glycyrrhizic Acid Nanoparticle against Multi-Drug Resistant K. pneumoniae with Genotypic Analysis

HAMDY AHMED EL-KHATEEB, Mahmoud Abd El-Mongy, Amal Sabry Othman

  •  Received: 15 December 2017
  •  Accepted: 28 February 2018
Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae can resist many antibiotic groups as they contain resistant genes responsible for multi-drug resistance. the study aimed to detect and identifying multi-drug resistant (MDR) K. pneumoniae from different clinical samples with detection and sequencing of both TEM and SHV genes, and using Glycyrrhizic acid nanoparticle as a treatment agent for MDR, ESBL producing K. pneumoniae strains. Clinical specimens were identified and confirmed by API-20E kit. ESBL producing strains were detected by double disk synergy test. TEM and SHV genes responsible for MDR in K. pneumoniae were detected by polymerase chain reaction (PCR) and sequence alignment was done using DNA sequencer. The effect of different concentrations of Nano Glycyrrhizic acid was detected with determination of minimum bactericidal concentration. 53.3% of the isolates were identified as K. pneumoniae. Males were highly infected than females. 45% K. pneumoniae isolates were multi-drug resistant containing ESBL enzyme. 52.8% of the MDR isolates harbored TEM gene, 72.2% isolates harbored SHV gene while 25% of the MDR isolates harbored both genes. Sequence alignment of both genes showed some mutations ranged between Deletion, Substitution and Insertion. Chloramphenicol was found to be the drug of choice to overcome multi-drug resistant ESBL producing K. pneumoniae with inhibition of 97.2%. The antibacterial activity of Nano Glycyrrhizic acid revealed that 10% concentration was the minimum bactericidal concentration (MBC) of the MDR isolates.

Keywords: Klebsiella pneumoniae, Glycyrrhizic acid, Multidrug-resistance and Extended spectrum β-lactamase