Typhoid fever is an infectious disease that affects millions of people around the world. A recipe based on Carica papaya, Cocos nucifera and Persea americana is used in traditional medicine in Togo against this disease. The aim of this study was to evaluate the phytochemical properties, and antimicrobial activities of the recipe of plant used in traditional medicine in Togo against Typhoid fever. Qualitative tests based on characterization reactions was use to identify phenolic compounds, alkaloids, terpenes and sterols, anthocyanins, reducing sugars, cardiac glycosides in the hydroethanolic extract. The Folin-Ciocalteu reagent reduction method was used to determine the total polyphenol content while, the Butanol-Hcl method was used to determine the proanthocyanidol content. Antimicrobial activity was evaluated using the 96-well plate microdilution method. The results showed that the hydroalcoholic extract of the recipe is rich in phenolic compounds 0.86±0.002 mgAGE/g. The different plant organs have a content of phenolic compounds between 0.31±0.006 and 0.52±0.005 mgAGE/g. Condensed tannin contents varied between 0.21%±0.001 and 0.35%±0.003 mgCE/g. The antiradical activity is between 0.069±0.007 and 0.074±0.000 mgAAE/g. The recipe is more active on hospital strains with an MIC of between 6.25 and 12.5 mg/ml. The MIC of plant organs is between 25 and 100 mg/ml.

Keywords: Carica papaya, Cocos nucifera, Persea americana, typhoid fever, Togo