African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 18 July, 2011; 5(14)

July 2011

Effects of UV irradiation on plants

Global change will definitely introduce changes in agricultural ecosystems that will affect plant productivity. However, the effects on plants will be different for each region depending on the pre-existing climatic conditions and the adaptation potential of local cultivated species. The solar energy from the sun is essential to support the life on our plant, via the process of photosynthesis. However a small proportion...

Author(s): Eissa Piri, Mahdi Babaeian, Abolfazl Tavassoli and Yasser Esmaeilian

July 2011

The in vitro efficacy of a Zimbabwean isolate of Zoophthora radicans Brefeld (Batko) in the control of Lepidoptera larvae infesting Brassica sp.

The use of microbes in pest control is an important aspect of integrated pest management (IPM). A Zimbabwean isolate of the fungal entomopathogen Zoophthora radicans was tested for efficacy in the control of Brassica infesting lepidopteran larvae. Z. radicanscaused 98.68%...

Author(s): W. Manyangarirwa, G. W. Zehnder, G. S. McCutcheon, P. H. Adler, J. P. Smithand A. N. Mphuru

July 2011

The antifungal mechanism of Bacillus subtilis against Pestalotiopsis eugeniae and its development for commercial applications against wax apple infection

The inhibition zones of three Bacillus subtilis isolates, BS-99, BS-23857 and BS-33608 against Pestalotiopsis eugeniae were, 13.5, 0, and 0 mm, respectively. BS-99 showed the strongest inhibitory activity, whereas, no inhibitory activity was reported for BS-23857 or BS-33608. The spore germination rates of P. eugeniae were not significantly inhibited...

Author(s): Hsiu Fen Lin, Tsang Hai Chen, and Shan Da Liu

July 2011

The effect of fertilizer amendment on diesel biodegradation in contaminated soils

Diesel biodegradation and the effect of application frequency were investigated in artificially contaminated soils (loam soil and sea sand) amended with commercial fertilizers and the effect of application frequency. Two sets of four equal portions (2 kg each) of diesel-contaminated soils were amended with 10% (w/w) of 2 different commercial fertilizers at 4 different but regular intervals for up to four times during a...

Author(s): D. Padayachee and J. Lin

July 2011

Better criterion screening for left ventricular hypertrophy by electrocardiogram with different purposes

According to the recommendations for the standardization and interpretation of the electrocardiogram (ECG) of AHA/ACCF/HRS declared in 2009, there are more than 30 criteria for diagnosing left ventricular hypertrophy (LVH). The sensitivity of the various criteria is generally quite low. However, the specificity of ECG in LVH could be 85 to 90%, acting as a strong predictor for cardiovascular events. To...

Author(s): Ai Song, Tian Chang Li, Ning Li Wang, Yuan Bo Liang, and Yi Peng

July 2011

Evaluation of inoculation of plant growth-promoting rhizobacteria on cadmium and lead uptake by canola and barley

Plant growth promoting bacteria are important for plant growth and heavy metal uptake of metal-polluted soils. In this study, to isolate (cadmium and lead) Cd and Pb-resistant bacteria, seven strains of Pseudomonas putida and Pseudomonas fluorescens were plated onto Tryptone Soya Broth supplemented with different concentrations of Pb(No3)2and CdCl2 (0, 30, 60 mg L-1). Three heavy metals...

Author(s): Jila Baharlouei Yancheshmeh, Kazem khavazi, Ebrahim Pazira and  Mahmood Solhi

July 2011

Agricultural utilisation of dairy sewage sludge: Its effect on enzymatic activity and microorganisms of the soil environment

Biochemical indices related with the transformations of C, N and P are frequently used in the diagnosis of soil quality. Such an assessment usually includes analysis of the following enzymes – the activity of dehydrogenases and of hydrolytic enzymes such as phosphatases, urease and protease. The objective of the study presented here was evaluation of dairy sewage sludge as an agent causing an improvement of soil...

Author(s): Magdalena FrÄ…c and Stefania Jezierska-Tys

July 2011

Prevalence of Leptospira hardjo antibody in bulk tank milk in some dairy herds in Mashhad suburb

Bulk tank milk samples were collected from 47 dairy herds in Mashhad suburb. From 19 herds, one sample was collected at each season in 2009 and from the rest of the 28 dairy herds one sample was collected only at autumn, 2009 and samples was tested by ELISA method for detecting antibodies to Leptospira interrogans serovar hardjo. These herds, which had not been vaccinated against leptospirosis within...

Author(s): Elias Tabatabaeizadeh, Gholamreza Hashemi Tabar, Nima Farzaneh and Hesam A. Seifi

July 2011

Direct detection of Escherichia coli O157 and its major virulence factor genes in animal faeces at slaughter using multiplex polymerase chain reaction (PCR)

Although more than 100 serotypes of shigatoxigenic Escherichia coli (STEC) have been implicated in cases of human diseases, E. coli O157 is the most common serogroup connected with sporadic cases and large outbreaks of diseases in many countries. Rapid and sensitive identification of this dangerous pathogen is important for patient management and for prompt epidemiological investigations. PCR...

Author(s): Khandaghi Jalil, Razavilar Vadood and Barzegari Abolfazl

July 2011

Determination of an economical medium for growth of Clostridium butyricum TK2 using orthogonal test

Clostridium butyricum is a widely utilized probiotics, also used as an alternative to antibiotics for humans and growth promotion in a wide variety of livestock species. The objective of this study is to develop an economical and practical fermentation medium for the growth of C. butyricum TK2 using orthogonal test. The optimal fermentation medium was obtained by the single factor and the orthogonal test...

Author(s): HaiKuan Wang, AnDong Li , FeiFei Liu and Wei Qi

July 2011

Prevalence of dental caries bacterial pathogens and evaluation of inhibitory concentration effect on different tooth pastes against Streptococcus spp

Dental caries is a microbiologic infection of the tooth which results in localized dissolution and destruction of the calcified tissue and it is one of the most common chronic infectious diseases in humans throughout the world. People spent huge amounts of money and time in treating dental caries. Hence, it is essential to prevent and control dental caries in public health. In our studies, a total of 70 clinical...

Author(s): A. Maripandi, Arun Kumar T. and Ali A. Al Salamah

July 2011

Aerobic composting of water lettuce for preparation of phosphorus enriched organic manure

In this study, rock phosphate (RP-compost) enriched composts were prepared by mixing rock phosphate with water lettuce (Pistia stratiotes) with and without effective microbes. The treatment comprised of four types and includes control plant material (water lettuce), water lettuce and rock phosphate (WL+RP), water lettuce, rock phosphate, effective microorganisms (WL+RP+EM) and water lettuce, rock phosphate, effective...

Author(s): Sadia Kanwal, Shazia Iram, Matiullah Khan and Iftikhar Ahmad

July 2011

Single multiplex PCR assay to identify the shiga toxin

Strains of shiga toxin–producing Escherichia coli (STEC) and Shigella dysenteriae type1 have been associated with outbreaks of diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS) in humans. Most clinical signs of disease arise as consequence of the production of shiga toxin/shiga toxin 1 (Stx/Stx1), shiga toxin 2 (Stx2) or combination of these toxins. Here, we designed a...

Author(s): Eisa Tahmasbpour Marzony, Mahdi Kamali, Mojtaba Saadati, Amir Homayoun Keihan, Abbas Ali Imani Fooladi, and Sharareh Sajjadi  

July 2011

Virus infection disturbs cyclin expression, leading to cell cycle arrest in the unicellular marine algae Emiliania huxleyi and Chrysochromulina ericina

To investigate the influences of viral infection on cell cycle of marine algae, we examined the changes of expression and activity of G2/M-phase cell cycle regulators in two unicellular marine algae Emiliania huxleyi and Chrysochromulina ericina after viral infection by immunoblot. The results revealed that the expression of cyclins could be altered by viral infection, thus inhibiting the progression...

Author(s): Jingwen Liu, Tianling Zheng, Gunnar Bratbak and Runar Thyrhaug  

July 2011

The respiratory syncytial virus may not always be responsible for Bronchiolitis in children

The aim of this study is to investigate the viral agents and the viral profile in children under 5 years of age who are referred to our hospital due to acute wheezing. The nasal smears of 55 children who were referred to Kagithane State Hospital from now on (KSH) with acute wheezing between November 2009 and March 2010 were analyzed with multiplex PCR and the distribution of respiratory virus detected in these smears...

Author(s): Gülsen Meral Sezer, Emel KabakoÄŸlu Ünsür, Semra KayaoÄŸlu and Faruk Akçay

July 2011

Haematological parameters in blood of maedi-visna virus-infected and uninfected sheep

The maedi-visna virus (MVV) classified as a lentivirus of the retroviridae family, causes a very common economically important disease in sheep, in many parts of the worlds. Presences of the infection in Turkey have been shown by researches in previous studies. In this study all blood samples were examined by ELISA and PCR to detect MVV antibody and antigen responses, respectively. Hematological findings were...

Author(s): Serkal Gazyagci, Ahmet Kursat Azkur, and Muhammet Eren Aslan

July 2011

Biochemical and molecular characterization of hemolytic Bacillus licheniformis strains isolated from shrimp and clam aquacultures

A polyphasic approach was used to discriminate among the significantly hemolytic and closely related Bacillus licheniformis strains, NE-1, NE-2 and NE-3. Utilization of the API 20E kit revealed that B. licheniformis NE-1 is unique in its ability to utilize sorbitol as a sole carbon source, whereas B. licheniformis NE-2 is an exceptional in L-arabinose utilization. The phylogenetic analysis...

Author(s): Nermeen A. ElSersy and Eman A. H. Mohamed

July 2011

Trends of sudden wilt syndrome in sesame plots irrigated with delayed intervals

In this work, sudden wilt syndrome in sesame was investigated during varying irrigation treatments. For the four irrigation intervals numbers of infected plants and infected rows were determined in experiments conducted during growing periods. The resulting data were transformed to percent values and regressed against the irrigation intervals. The correlations between the rates of infected plants and irrigation...

Author(s): Hamit Kavak and Erkan Boydak

July 2011

Perpetration of styrene–butadiene acrylate anti bacterial binders and their application on cotton/polyester printing

This paper reports preparation of four samples of styrene – acrylic emulsion copolymers. It was performed in the presence of acrylic- acid, acryl amid, ammonium persulfate as a free radical initiator and sodium dodecyl benzene sulfate as an anionic emulsifier at 80°C. The resulting Ag nanoparticle-loaded grafted fabric was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron...

Author(s): Hossein Najafi Kutanaee

July 2011

Enrichment and diversity analysis of the thermophilic microbes in a high temperature petroleum reservoir

Thermophilic microbial diversity in production water from a high temperature, water-flooded petroleum reservoir of an offshore oilfield in China was characterized by enrichment and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Six different function enrichment cultures were cultivated one year, at 75°C DGGE and sequence analyses of 16S rRNA gene fragments were used to assess...

Author(s): Guihong Lan, Zengting Li, Hui zhang, Changjun Zou, Dairong Qiao and  Yi Cao

July 2011

Screening of lactobacilli bacteria isolated from gastrointestinal tract of broiler chickens for their use as probiotic

A study was conducted to isolate lactobacilli from the gastrointestinal (GI) tract of broiler chickens and to evaluate their application as a probiotic supplement. The GI contents of 12 broiler chickens from three different ages were collected and cultured on Man Rogosa and Sharp (MRS) agar. In order to determine bacterial species, different grown colonies were separated and subjected to the sequencing of the 16s...

Author(s): R. Majidzadeh Heravi, H. Kermanshahi, M. Sankian, M. R. Nassiri, A. Heravi Moussavi,  L. Roozbeh Nasiraii and A. R. Varasteh

July 2011

Development and application of a novel multiplex polymerase chain reaction (PCR) assay for rapid detection of various types of staphylococci strains

In this study, a novel multiplex- polymerase chain reaction (PCR) for rapid detection of various staphylococci strains, including methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive Staphylococcus aureus, (MSSA) methicillin-resistant coagulase-negative staphylococci (MRCNS), methicillin-sensitive coagulase-negative staphylococci (MSCNS) and non-staphylococci...

Author(s): Zhenbo Xu, Lin Li, Xihong Zhao, Jin Chu, Bing Li, Lei Shi , Jianyu Su and Mark E. Shirtliff,

July 2011

Effect of neem (Azadiracta indica) aqueous extract on Casuarina equisetifolia seed germination, growth of Frankia and some rhizospheric microorganisms

Aqueous neem (Azadiracta indica) leaf extract was tested for its effect on Casuarina equisetifolia seed germination capacity, growth of Frankia and on some dominant rhizosphere microorganisms under casuarinas grown in soil contaminated with sewage water. Analysiswas carried out to determine the main micro- and macroelement content of different extract amounts. The addition of...

Author(s): Sayed W. F., Salem W. M. and Ali M. S.

July 2011

The in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus from hospital currency notes

We determined the in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus isolates and presumptive roles of plasmid DNA using standard microbiological procedures. A total of 32 paper naira notes from seven different hospital sections (mostly from the patients) were analyzed. Thirteen notes (40.6%) gave isolates of S. aureus and subsequent resistant range of between 62.5...

Author(s): ADEGOKE, Anthony A. and OKOH, Anthony I.

July 2011

Study on productivity of epilithic algae in Urumqi River Basin in Northwest China

The primary objective of this study was to make a deep investigation on the concentration of chlorophyll a and ash-free dry mass (AFDM) estimates for algae growing on stones in Urumqi River located in an arid region of northwest China. The study was conducted on three sites. A highly improved method for measuring primary productivity in situ was used to estimate the primary production of algae...

Author(s): Li Lanhai, Mupenzi Jean de la Paix, Chen Xi, Achal Varenyam, Bao Amning and Habiyaremye Gabriel

July 2011

Effect of jujube extract on oxidative injury in heart muscles of exhausted training rats

Jujube is an important plant in traditional Chinese medicine and is recommended for the treatment of some diseases such as tumors and cardiovascular disease related to the production of radical species resulting from oxidative stress. The objective of this study was to investigate effect of jujube extract on oxidative injury in heart muscles of exhausted training rats. Four groups of animals were studied: (a) sedentary...

Author(s): Sun Liang and Jiang Juan

July 2011

Prevalence and antimicrobial resistance profiles of Listeria monocytogenes in spontaneous abortions in humans

Listeria monocytogenes is a causative agent of listeriosis. Clinical manifestation of invasive listeriosis is usually sever and include sepsis and meningoencephalitis. Uterian infection in listeriosis depend on gestational age varies from preterm labour, delivery of infected neonate to abortion. The present study investigated the incidence and antibiograms of the organism in clinical samples were collected from...

Author(s): Lida Lotfollahi, Jamileh Nowrouzi, Gholamreza Irajian, Faramarz Masjedian, Bahram Kazemi, Laleh Eslamian Arash Falahat and Maryam Ramez

July 2011

Bacteriological study of vaginal discharge of pregnant women using Gram stain smear and culture

Bacterial vaginosis is caused by an imbalance of the organisms that naturally exist in the vagina. The importance of bacterial vaginosis with respect to pregnant women’s health is emphasized by the association betweenbacterial vaginosis and adverse outcome of pregnancy. The aim of present study was to evaluate the direct smear microscopy and culture for determination of bacteria from...

Author(s): Manigeh Mehdinejad, Azar Dokht Khosravi, Hamideh Yazdizadeh and Porandokht Afshari

July 2011

Detection of the anaerobic bacteria in the odontogenic cyst fluids using polymerase chain reaction (PCR) method

Odontogenic cysts are slow growing lesions which are formed by epithelium. They may reach to a substantial size without symptoms for a long time. Radicular cysts’ (RCs)  and odontogenic keratocysts’ (OKCs)  are common odontogenic cysts of jaws. The main purpose of this study is to evaluate if anaerobic bacteria play a role in the pathogenesis of the...

Author(s): Fahriye Keskin, Sevgi Ciftci, Sirmahan Cakarer, Firat Selvi, Taylan Can, Sevda Ozel and Cengizhan Keskin

July 2011

An investigation on the pollen germination and tube growth in some Prunus persica genotypes and cultivars

Peaches and nectarines (Prunus persica) are stone fruit trees which their pistils needed to be pollinated and fertilized to set fruits therefore; pollen characteristics including pollen viability, longevity, morphological homogeneity, germination and pollen tube growth rate are very important component of fertilization and fruit setting. However, study of main pollen traits is one of the most important...

Author(s): Yavar Sharafi

July 2011

Mutational studies for hyper producers of L-glutamic acid from Bacillus strains UG1 and OG4 from vegetable proteins

  An investigation was carried out to produce mutant isolates from Bacillus subtilis UG1 and Bacillus licheniformis OG4 isolated from vegetable proteins using a chemical mutagen N-methyl-N-nitrosoguanidine (NTG) at dosage concentration of 250 µg/ml. The mutant isolates obtained from wild strain of B. subtilis UG1 produced mutants (M1 to M9) and was screened for hyper...

Author(s): Lawal, A. K., Oso B. A., Sanni, A. I. and Akinola, S. O.