April 2012
Diversity of NosZ gene in three municipal wastewater treatment plants located in different geographic regions
Diversity of nitrous oxide reductase genes (NosZ) in activated sludge was compared in the biological treatment process (BTCs, anaerobic, anoxic and aerobic process) of three municipal wastewater treatment plants located in different geographic regions. A total of 18 specific bands generated after polymerase chain reaction- denaturing gradient gel electrophoresis (PCR-DGGE) analysis. The diversity of NosZ in MWTPs of...
April 2012
New insight into the regulation of classâ…¡ bacteriocin production by quorum sensing in Lactobacillus pentosus 31-1
The biosynthesis of class II bacteriocins is frequently regulated by a quorum-sensing regulatory mechanism meditated by autoinducing peptide. Here, it was preliminarily confirmed that bacteriocin production by Lactobacillus pentosus 31-1 was controlled by a quorum sensing (QS) system meditated by peptide signal molecules in the cell-free supernatant (CFS). Effects of the growth medium and inoculums size...
April 2012
Biological potential of Pseudomonas sp. BS1 in the control of Phytophthora root rot of soybean
In this study, the biological potential of Pseudomonas sp. BS1 in the control ofPhytophthora root rot of soybean was investigated. Fermentation filtrates ofPseudomonas sp. BS1 inhibited zoosporangium formation and zoospore germination ofPhytophthora sojae (P. sojae), and the inhibitory effect gradually weakened with the increasing filtrate dilution. It had a relatively strong...
April 2012
Isolation of lectin and lectin derivatives from Haworthia limifolia: An in-vitro investigation
The ability for medicinal plants to aid in wound healing lends itself to cell-agglutinating and sugar-specific protein. These are collectively termed lectins. Although, the function of these lectins in plants are limiting, the benefit to human in terms of wound healing is of vital importance. With many new infections arising as a result of bacteria, fungi and viruses developing immunity against western medicines, the...
April 2012
Iranian traditional medicine: Comparison of the antibacterial effect of ANNAS 0.2% and chlorhexidine 0.2%
Anbarnesa smoke have a long history of antimicrobial effects in Iranian traditional medicine. The present study compared the effect of ANNAS (extract of Anbarnesara smoke) mouth wash 0.2% and Chlorhexidine 0.2% against some bacterial species. In this experimental study, the specific culture mediums were used for each species and the antibacterial efficacies of the mouth rinses were determined by means of agar diffusion...
April 2012
Optimization of the culture conditions of a Chlamydomonas high oil content ultraviolet mutant CC124-M25 and polymorphism analysis by inter-simple sequence repeat (ISSR)
Microalgal oils have been considered as a promising feedstock for biodiesel production, which can potentially completely substitute fossil fuels. In the present study, 4500 mutants were obtained from Chlamydomonas reinhardtii CC124 through ultraviolet (UV) induced mutagenesis. After screening these mutants using HSM and HSM-N media, a mutant CC124-M25 with high oil content was selected under an HSM-N medium...
April 2012
In vitro synergy profile of some antibiotics against mono and multidrug resistant clinical isolates of Pseudomonas aeruginosa
It is becoming highly challenging for scientific community around the world to battle against rapidly increasing deadly superbugs. Because of heavy exposure of antibiotics and inadequate regimen against infectious organisms, antibiotic resistance in these pathogens has created an immense problem worldwide. Combined antibiotic therapy is frequently recommended for the treatment of such problems. This study is focused to...
April 2012
Influence of substrate concentration in accumulation pattern of poly(R) hydroxyalkonoate in Pseudomonas putida SU-8
Polyhydroxyalkanoates (PHAs) are accumulated by many bacteria intracellularly as carbon and energy reserves in presence of excess carbon source and limited nitrogen or other nutrient sources. The accumulation pattern of these PHA may vary as the supplements are varied. In this study, Pseudomonas putida SU-8 was allowed to grow in glucose and lactose containing medium, the organism was found to accumulate more...
April 2012
Prevalence of Hepatitis B detected through RT PCR in Khyber Pakhtunkhwa
Pakistan is highly endemic for Hepatitis B virus. Many studies have shown that 32% of patients with liver diseases are suffering with hepatitis B. Objective: The present project was designed to check the prevalence of Hepatitis B in those peoples of Khyber Pakhtunkhwa who visited a specific laboratory for diagnosis. The method included a four months data of Hepatitis B positive patients from January 2011 to April 2011,...
April 2012
Comparison of two mechanical instrumentation techniques in reducing root canal bacterial population
Microorganisms and their products play an important role in the pathogenesis of pulpal and periradicular diseases, so their elimination from root canal system as well as reinfection prevention are the main purpose of root canal treatment. This study was designed to compare the in-vitro efficiency of the Hero and M-Two rotary instruments in intracanal bacterial reduction. Fifty six human extracted teeth were...
April 2012
Comparative study on decolorization of reactive dye 222 by white rot fungi Pleurotus ostreatus IBL-02 and Phanerochaete chrysosporium IBL-03
White rot basidiomycetes have unique ability to decolorize synthetic industrial dyes. Initial experiment was performed with five locally isolated indigenous white rot species fungi S.commune IBL-01 (SC), P. ostreatus IBL-02 (PO) P. chrysosporium IBL-03 (PC), T.versicolor IBL-04 (TV) and G. lucidum IBL-05 (GL), for the selection of white rot fungal cultures...
April 2012
Identification of indigenous Xanthomonas isolates through 16S rRNA gene amplification and SDS PAGE analyses
Characterization of seven Xanthomonas strains comprising four species was done using molecular techniques, that is, 16S rRNA gene amplification and SDS-PAGE analysis. 16S rRNA gene amplification was performed to confirm the identity of strains. The primer sets used in the present study amplified 0.352 Kb and 1.3 Kb sized fragments of rRNA gene in all the strains. The species of the...
April 2012
Ecological studies on nitrogen fixing bacteria from leguminous plants at the north of Jordan
The rates of nitrogen fixation by symbiotic rhizobia are often two to three times higher than the ones exhibited by free-living nitrogen-fixing bacteria in soil. In this study, Rhizobium leguminosarum, Rhizobium meliloti, Rhizobium loti and Rhizobium ciceri were isolated and identified from leguminous plants grown at the north of Jordan. The aim of this study was initially to determine the...
April 2012
Detection of Salmonella, Clostridium perfringens and Escherichia coli from fecal samples of captive animals at the National Zoological Gardens of South Africa
Escherichia coli, Clostridium perfringens and Salmonella spp. are enterotoxic, ubiquitously distributed bacteria. Diseases caused by these pathogens are well recognized in humans, livestock, companion and zoo animals and can result in morbidity, mortality, and significant economic losses. The aim of the study was to identify and simultaneously amplify enterotoxigenic E....
April 2012
Evaluation of antibacterial properties and biochemical effects of monoterpenes on plant pathogenic bacteria
The antibacterial activity of twelve monoterpenes, namely camphene, (R)-camphor, (R)-carvone, 1,8-cineole, cuminaldehyde, (S)-fenchone, geraniol, (S)-limonene, (R)-linalool, (1R,2S,5R)-menthol, myrcene and thymol was tested against two plant pathogenic bacteria Agrobacterium tumefaciens and Erwinia carotovora var. carotovora using agar dilution method. For a better understanding...
April 2012
The T3S effector XopXccN of Xanthomonas campestris pv. campestris is involved in plant defense through interference with photosystems, reactive oxygen species (ROS) generation, and callose deposition
XopXccN and XopN are important type III secreted (T3S) virulence effector proteins ofXanthomonas campestris pv. campestris (Xcc) and Xanthomonas campestris pv.vesicatoria (Xcv), respectively. They were placed in the same T3S effector class based on sequence homology. It has been demonstrated that Xcv XopN suppresses pathogen-associated molecular pattern-triggered immune responses...
April 2012
Antimicrobial effect of amphotericin B electronically-activated water against Candida albicans
The activation of water by physical means stimulates a new scientific approach to microbiology, in particular, antimicrobial methods. However, many of these methods are unproven or have not been properly tested. Since the 1980s, a promising procedure known as biophysical-information therapy or bioresonance therapy (BRT) has emerged as an alternative method against microbial diseases, but it has not yet been properly...
April 2012
Inhibitory influence of biocontrol agents, plant oils, and an inorganic salt on Mycosphaerella fijiensis and Cordana musae
The search for alternatives to chemical treatments to control the causal pathogens of black sigatoka and Cordana leaf spot in banana was studied in vitro. We examined the effect of biocontrol agents, plant oils, and an inorganic salt on the mycelium growth ofMycosphaerella fijiensis and Cordana musae for 10 weeks. Trichoderma harzianumDGA01 and Bacillus...
April 2012
Susceptibility pattern among pulmonary and extrapulmonary isolates of Mycobacterium tuberculosis in north India
Drug resistant tuberculosis (TB) is an increasing problem worldwide. We retrospectively identified Mycobacterium tuberculosis isolates from treatment failure pulmonary TB (362),and extrapulmonary TB (338) patients (18-70 years) during a 4 year period (June, 2002-July, 2006). Drug susceptibility testing was carried out for the first line anti-tuberculosis drugs using standard proportion method. Of the...
April 2012
Screening of rhizobacteria against Fusarium oxysporum f. sp. melongenae, the causal agent of wilt disease of eggplant
Rhizosphere isolates of Bacillus and Pseudomonas species isolated from the roots of eggplants (Solanum melongena L.) were collected in eggplant cultivated areas in various geographical districts of Turkey. Isolates were evaluated for siderophore, protease and cyanide production along with the cell wall degrading enzyme production as well as phosphate solubilizing and nitrogen fixing...
April 2012
The effects of aluminum stress on bacterial community diversity in acidic red soils by polymerase chain reaction (PCR)-amplified restriction fragment length polymorphism
The soil bacterial communities in response to aluminum (Al) treatments were evaluated by polymerase chain reaction-amplified restriction fragment length polymorphism (PCR-RFLP) of 16S rRNA genes in acidic red soil samples in this study. A total of 6 bacterial communities were sampled from two representative soil types under different Al-treated concentrations. Bacterial genomic DNA was extracted and nested PCR-amplified...
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