African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 23 March, 2012; 6(11)

March 2012

Preliminary study on the epidemiology of bovine tuberculosis in Jimma town and its surroundings, Southwestern Ethiopia

A cross-sectional study was conducted from October 2007 to April 2008 on 384 dairy cattle in Jimma Town and 1,102 adult cattle slaughtered at Jimma abattoir. The objective of the study was to generate preliminary epidemiological data on bovine TB both in dairy and beef cattle in Jimma and its area. Simple random sampling, single intradermal tuberculin (SIDT) and comparative intradermal tuberculin (CIDT) tests of dairy...

Author(s): Tigre W., Alemayehu G., Abetu T. and Ameni G.

March 2012

Influence of pH and temperature on the production of staphylococcal enterotoxin H

The effect of pH was studied using lactic, acetic and hydrochloric acids plus sodium hydroxide on the production of staphylococcal enterotoxin H (SEH) in various strains ofStaphylococcus aureus. Furthermore, influences of temperatures were examined. The lowest pH at which SEH was still detected was dependent not only on the acid used but also on the given strain. The smallest inhibitory effects on the production of SEH...

Author(s): Jana Kristlová, Petra JiÅ™íčková and Jarmila VytÅ™asová

March 2012

Aflatoxigenic potential of Aspergillus spp. isolated from groundnut seeds, in Burkina Faso, West Africa

Seven strains of Aspergillus spp. have been screened for their ability to produce aflatoxins in coconut broth media. Three were local isolates and four were reference strains obtained from the United States Department of Agriculture (USDA) and Centre for Disease Control and Prevention (CDC) Atlanta and used as positive and negative controls. All strains were previously cultivated...

Author(s): Pane B. OUATTARA – SOURABIE, Philippe A. NIKIEMA, Nicolas BARRO, Aly SAVADOGO and Alfred S. TRAORE

March 2012

Antimicrobial resistance patterns and emerging fluoroquinolone resistant Salmonella isolates from poultry and asymptomatic poultry workers

Non-typhoidal Salmonellae have been extensively incriminated worldwide as common causes of bacterial gastroenteritis in humans and animals. This study investigated patterns of resistance of prevailing Salmonella serotypes from poultry and asymptomatic poultry workers. A total of 560 stool samples, comprising 200 from non-diarrhoeic workers and 360 from diarrhoeic poultry were randomly collected. Samples were...

Author(s): Fasure A. K., Deji-Agboola A. M. and Akinyemi K. O.

March 2012

Antimicrobial resistance of Staphylococcus aureus isolated from human and food against Linezolid, Quinupristin-Dalfopristin, Quinolones and Imipenem

Staphylococcus aureus is a versatile pathogen of humans and animals that has evolved resistance to all classes of antimicrobials. A total of 293 S. aureus isolates including 71 obtained from human and 222 recovered from food against crucial antimicrobial agents used in human and veterinary practice were examined for the antimicrobial resistance profiles. The in vitro activities of new agents...

Author(s): Seza Arslan and Fatma Özdemir

March 2012

An extracellular lipase from the endophytic fungi Fusarium oxysporum isolated from the Thai medicinal plant, Croton oblongifolius Roxb.

From 65 endophytic fungal isolates, ten were found to produce extracellular lipase activity, with Fusarium oxysporum isolate PTM7, isolated from the leaves of Croton oblongifoliusRoxb. (Plao yai), yielding the highest level. The lipase activity in the basal culture medium of PTM7 was highest with 1% (v/v) olive oil, 1% (w/v) peptone and 0.5% (w/v) sodium nitrate as the carbon, organic and...

Author(s): Tuangporn Panuthai, Prakitsin Sihanonth, Jittra Piapukiew, Sarintip Sooksai, Polkit Sangvanich and Aphichart Karnchanatat 

March 2012

Luminal hindgut bacterial diversities of the grass and sugarcane feeding termite Trinervitermes trinervoides

Termites depend on intestinal fauna for digestion of lignocellulose, but the variations in gut bacterial diversity between termites species is poorly characterised. Bacterial diversities from the third proctodeal hindgut segments (P3, the paunch) of the grass and sugarcane feeding Trinervitermes trinervoides (Termitidae: Nasutitermitinae) termites were compared using 16S rRNA gene pyrosequence analysis. A...

Author(s): Tendai Walter Sanyika, Konanani Justice Rashamuse#, Fritha Hennessy and Dean Brady, # Author contributed equally to the work.

March 2012

Antibiotic susceptibility of bacterial pathogens isolated from food preparation areas in hospice kitchens

The emergence of bacterial antimicrobial resistance in both medical and agricultural fields has become a serious problem worldwide. The implication is that greater emphasis needs to be placed on preventative strategies such as hygiene, rather than relying solely on antibiotic therapy, and that these strategies should be developed in healthcare settings such as hospices and also in the community. Samples from food...

Author(s): J. Nkhebenyane, M. M. Theron, P. Venter and J. F. R. Lues

March 2012

Serological prevalence of ovine and caprine chlamydophilosis in Riyadh region, Saudi Arabia

The serological prevalence of Chlamydophila abortus was determined in 399 sheep and 171 goats in Riyadh region, Saudi Arabia, using the CHEKIT enzyme-linked immunosorbent assay (ELISA). Sera from 30 (7.52%) sheep and 59 (34.50%) goats were positive for anti-C. abortus antibodies. Higher serological prevalence and antibody concentration (percent optical density) was recorded in goats as compared to sheep....

Author(s): Riyadh S. Aljumaah, and Mansour F. Hussein

March 2012

Effects of long-term fertilization on nifH gene diversity in agricultural black soil

Black soil is a precious resource: it is an important grain-base commodity and a highly fertile soil. The long-term use of chemical and organic fertilisers has affected the microbial community and agricultural sustainability of black soil. We used nitrogenase reductase (nifH) as a biomarker of soil-borne nitrogen-fixing microbes to study the effects of long-term fertilisation on a field. Fertilisation started in...

Author(s): Hui Tang,, Miao Yu, Yanyuan Wang, Xiaowan Han, Xiaogai Wang, Wei Jin, Fengqin Chi and Dan Wei

March 2012

The diagnosis and surgical treatment of pulmonary fungal infections

The clinical signs, diagnosis, and surgical management experiences of 51 cases of pulmonary fungal infections were retrospectively summarized. The clinical data were taken from 51 pulmonary fungal infection patients treated in the Thoracic Surgery Department of the Chinese PLA General Hospital from 1981 to 2008. This case series included 3 cases of Candida albicans, 22 cases of Aspergillus, and 26 cases...

Author(s): Yu-Qi Wang, Yu-E Sun, Xiang-Yang Chu, Wei-Min Dai, Bo Yang and Ming Zhao

March 2012

Characterization and optimal production of alkaline α-amylase from Bacillus sp. DLB 9

The present study is concerned with the characterization and production of alkaline α-amylase from newly isolated Bacillus sp. DLB 9. Maximum enzyme synthesis occurred at 2nd day of incubation. Maximum amylase activity was observed at pH 9.0 and 60°C. Urea was found to be most inhibitory for the activity of amylase. Wheat bran was found to be a suitable natural source for maximum production of...

Author(s): Pardeep Kaur and Ashish Vyas

March 2012

Investigating the effect of boron spray on yield nutrient content, texture and brix index of apple (Sheikh Amir Variety) in Shirvan region

The influence of late-season boron (B) application on the bud B concentration and fruit set was studied in apple (Sheikh Amir Variety). The experiment was carried out on 10-year-old trees Shirvan region of Iran. In order to study the effect of boron on some characters of apple Sheikh Amir Variety fruits an experiment was conducted during 2010 and 2011 seasons cropping season in apple orchard at Shirvan region....

Author(s): Ahmad Asgharzade, Gholam Ali Valizade and Mahdi Babaeian

March 2012

Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by Serratia marcescens C9 isolated from activated sludge

In this study, an aerobic bacterial strain capable of utilizing di-n-butyl phthalate (DBP) as sole carbon source and energy was isolated from activated sludge collected from a plastic molding plant. Based on its morphology, physiochemical characteristics, and 16S rDNA sequence, the strain was identified as Serratia marcescens C9. The Taguchi method was used for determining the optimum condition of DBP...

Author(s): Chuang Li#, Xiuli Tian#, Zhengsuo Chen, Dong Yu, Jiayu Deng, Heng Xu

March 2012

Selection of Beauveria bassiana strains against the two spotted spider mite, Tetranychus urticae Koch in laboratory and greenhouse trials

South African strains (62) of Beauveria bassiana (Balsamo) Vuillemin were screened for pathogenicity against two spotted spider mite, Tetranychus urticae Koch, in laboratory bioassays on bean leaves, Phaseolus vulgaris L., under greenhouse conditions. In the first bioassay, strains of B. bassiana were applied at a single concentration of 107 conidia ml-1. A...

Author(s): M. C. Gatarayiha, M. D. Laing and R. M. Miller

March 2012

Bacterial community in the roots and rhizosphere of Hypericum silenoides Juss. 1804

Hypericum silenoides Juss. 1804 is an important medicinal plant used in Mexico, but the bacterial community associated with it is unknown. The bacterial communities in the rhizosphere and roots of H. silenoides were isolated and identified by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ribosomal types were determined by amplified ribosomal rDNA restriction...

Author(s): E. López-Fuentes, V. M. Ruíz-Valdiviezo, E. Martínez-Romero, F. A. Gutiérrez-Miceli, L. Dendooven and R. Rincón-Rosales

March 2012

Experimental study of IL-17 in mice infected with Mycoplasma suis

At present, only a few studies are available for the diagnosis of the T help cell 17(Th-17) about Mycoplasma suis. The objective of this study was to investigate the variation of interleukin-17(IL-17) in peripheral blood and spleen of M. suis-infected BALB/c mice. The variation of IL-17 were detected by using the method of ELISA, RT-PCR, Immunofluorescence double staining assays after infecting 1, 3,...

Author(s): TIAN Jing and BA Cai-feng

March 2012

Chitinase like activity of metabolites of Pseudomonas fluorescens Migula on immature stages of the mosquito, Culex quinquefasciatus (Diptera: Culicidae)

The present study describes the chitinolytic activity of extra cellular mosquitocidal proteins of Pseudomonas fluorescens and its involvement during pathogenesis in insects against larvae and pupae of Culex quinquefasciatus. The chitinolytic activity of purified extra cellular protein was assayed by using artificial colloidal chitin polymer substrates. The effect of purified protein in the...

Author(s): Usharani Brammacharry and Kummankottil Paily

March 2012

Heterogenic abilities in adhesion and invasion among clinical isolates of Porphyromonas gingivalis with Type II fimbriae

Porphyromonas gingivalis (P. gingivalis) fimbriae are classified into six types (Types I to V and Ib) based on the fimA genes encoding FimA (a subunit of fimbriae), and they play a critical role in bacterial adhesion to and invasion of gingival epithelium. Accumulated evidences suggest that P. gingivalis strains with Type II fimbriae (designated Pg-II) are more virulent than other...

Author(s): Li Gao, Di Miao, Shu Meng, Lei Zhao, Shujuan Guo and Yafei Wu

March 2012

The analysis of the impacting factors of probiotics on immune responses

  Probiotics are capable of enhancing immune responses, though their mechanisms have not been well understood. This study showed that probiotic strains and research objects are the most important impacting factors by analyzing the results of probiotics on immune responses. The immunomodulation of probiotics is related to the selected criteria, but the heterogenous strains may have good capacity. Although the...

Author(s): Zhipeng Li, Guangyu Li, Hanlu Liu, Jiaping Zhao, Yi Jing and Fuhe Yang

March 2012

Environmental and fungal contamination of palm oil sold in Anyigba Market, Nigeria

  In this present study, the environmental and fungal contamination of palm oil collected from Anyigba market, Nigeria was investigated. Results revealed the presence of 0.3% moisture, 0.2% impurity and 6.0% free fatty acid. These values were higher than the acceptable values for palm oil as recommended by the Nigerian Agency for Food and Drug Administration (NAFDAC) Research. Microbial content of the palm oil...

Author(s): S. C. Enemuor, S. A. Adegoke, A. O. Haruna and O. O. Oguntibeju

March 2012

Activation of enzyme phytase in Aspergillus niger LCWU 21 by surfactants

Present study describes the activation of enzyme phytase in Aspergillus niger LCWU 21 isolated from the soil by solid substrate fermentation (SSF) using wheat bran as culture medium. The addition of surfactants showed stimulatory effect on enzyme formation. Effect of the addition of surfactants showed that the addition of Triton x-100 enhanced enzyme production (60.3 U/ml). It was found that concentration and...

Author(s): Arifa Tahir

March 2012

Profiling the transcriptome of Sclerotium cepivorum Berk related to white rot on garlic (Allium sativum Linnaeus)

One concern for garlic (Allium sativum Linnaeus) producers is the damage caused by the pathogen Sclerotium cepivorum Berk. Unfortunately, the genetic and molecular mechanisms that participate during S. cepivorum Berk pathogenesis are currently unknown. In order to identify and isolate genes that are differentially expressed by the fungus during garlic white rot pathogenesis, PCR-based...

Author(s): Humberto R. Medina, Gerardo Acosta-García, Claudia Ivonne Muñoz-Sánchez, María Isabel Hernández-Álvarez, Mario Martín González-Chavira, Felipe Delgadillo-Sánchez, Irineo Torres-Pacheco, Ramón Gerardo Guevara-González and Lorenzo Guevara-Olvera

March 2012

Phenotypic characterization of seven Cercospora medicaginis isolates infecting annual Medicago species in Tunisia

Cercospora medicaginis is a widespread fungal pathogen that reduces yields and forage quality of annual and perennial Medicago species. The lack of studies on this pathogen has prompted us to characterize the morphology in culture and the aggressiveness of seven C. medicaginis isolates infecting annual Medicago species in different geographic area in Tunisia. The morphology...

Author(s): N. Djébali, N. Gaamour, M. Badri, M. Mrabet, Y. Badri, S. Elkahoui and M. E. Aouani

March 2012

Hygienic quality of raw milk at Sardi breed of sheep in Morocco

In Morocco, sheep farming is of particular socio-economic importance in the country’s supply of red meat. Moroccan sheep population is estimated at 16 million, head of which half are females. Among Moroccan breeds in high demand, Sardi is known for its very high productivity and meet good quality. The aim of  this study  is to evaluate the microbiological quality of raw milk from Moroccan Sardi sheep...

Author(s): Fatima Bouazza, Rachida Hassikou, Farida Ohmani, Jamila Hmmamouchi,  Jihane Ennadir, Aicha Qasmaoui, Zakaria Mennane,  Reda Charof and  Khadija Khedid

March 2012

Prevalence of vaginal candidiasis infection in diabetic women

Vulvovaginal Candidiasis (VVC) is a female genital system infection that occurs due toCandida species. It is estimated that as many as 75% of women experience at least one episode of Vulvovaginal Candidiasis during the lifetime. The aim of this study is to determine the prevalence rate of Vaginal Candidiasis infection in diabetic women referred to Kermanshah Diabetes Research Center (KDRC), Iran, in 2010. This...

Author(s): Reza Faraji, Mehr Ali Rahimi, Fatemeh Rezvanmadani and Masoud Hashemi

March 2012

Development status of Arbuscular mycorrhizal fungi associated with invasive plant Coreopsis grandiflora Hogg.

According to the distribution of Coreopsis grandiflora Hogg., samples were collected from two natural invasion sites; Jufeng and Beijiushui and cultivated site to research AM fungal arbuscule structure of C. grandiflora Hogg., colonization status in different sample plotsand altitudes, and the correlation between colonization status and soil factors in the...

Author(s): Bai Yanfang, Li Min, and Guo Shaoxia,

March 2012

Detection of Mycobacterium spp. by polymerase chain reaction in Nile tilapia (Oreochromis niloticus) in Campeche, Mexico

Mycobacteriosis is one of the serious diseases that affect a wide range of cultured fishes which is also dangerous for human. The aim of this study is to confirm the presence ofMycobacterium spp. that has been reported in 2007 in Campeche, Mexico. For this purpose, we evaluate fishes from a tilapia farm that previously reported clinical signs similar to Mycobacteriosis like granulomas. At first, the specific...

Author(s): Karina Y. Sonda-Santos and Maurilio Lara-Flores

March 2012

Physico-chemical growth requirements and molecular characterization of indigenous Spirulina

The present study was designed to explore the indigenous Spirulina and its mass cultivation by optimizing the physico-chemical growth requirements. One hundred and twenty samples were collected from different soils and water from three districts (Sargodha, Lahore and Faisalabad) of Punjab. Collected samples were shifted to laboratory immediately under sterile conditions to avoid contamination and were kept under dim...

Author(s): Muhammad Qasim, Imran Najeeb, Majeeda Rasheed, Khawar Ali Shahzad, Abdul Ahad, Zahida Fatima and Zubair Anwar

March 2012

Characterization of Brucella species in Mexico by Bruce-Ladder polymerase chain reaction (PCR)

The typing of 153 Brucella strains isolated from different regions of Mexico was performed by multiplex PCR Bruce-Ladder. Results showed that microbiological typing and multiplex Bruce-ladder amplification were identical for all Brucella isolates tested. Because of its speed, Bruce-Ladder PCR is a useful method in the typing of Brucella species isolated from animal and...

Author(s): Aurea Itzel Morales-Estrada, Joel Castillo-Salto, Ahidé López-Merino, María Rosario  Morales-García, Juan Gabriel Valle-Valdez and Araceli Contreras-Rodríguez

March 2012

Investigation and analysis on the adverse reactions caused by 12 kinds of commonly used traditional Chinese medicine injections

This study was aimed to explore the characteristics and rules of the adverse drug reactions (ADR) caused by the 12 kinds of traditional Chinese medicine injections and to provide reference for rational clinical drug use. Retrospective study methods are adopted to make statistical analysis on the cases of ADR caused by the 12 kinds of commonly used traditional Chinese medicine injections, such as Chuanhuning, Yanhuning,...

Author(s): Shuang-xing Hou#, Lian-fang Yuan#, Fang Du, Li Wang, Li-juan Liu, Xiao-gang Kang, Xue-dong Liu, Zhong-liang Wu, Gang Zhao and Yan-chun Deng

March 2012

Emergence of a novel GyrA variant from a strain of multidrug-resistant Klebsiella pneumoniae isolated from in China

A strain of Klebsiella pneumoniae (NB029) was positive for blaTEM (beta-lactamase gene), aph(3’)-â… (aminoglycoside modifying enzyme gene), gyrA (quinolone resistance gene), trbC (genetic marker of plasmids), tnp513, IS26, IS903 (genetic markers of transposons and insertion sequences), intâ… 1 (genetic marker of integrons), qacEâ–³1 (drug efflux gene) by PCR assays. So these positive genes confer...

Author(s): SHI Yu-bo, MI Zu-huang and WENG Xing-bei

March 2012

Etiology of children’s diarrhoea in Southern India: Associated pathogens and usual isolates

The frequency of diarrhoeagenic bacteria, isolated from all sampling areas showed thatEscherichia coli was recorded as the predominant bacterium with 58.57%, followed byVibrio, Salmonella, Aeromonas, Shigella, Pseudomonas, Streptococcus, Proteus andStaphylococcus with 40.58, 33.47, 30.54, 10.46, 10.04, 10.04, 7.11 and 5.43%, respectively. E. coli is the most...

Author(s): Subashkumar R., Tha. Thayumanavan, G. Vivekanandhan and P. Lakshmanaperumalsamy

March 2012

Comparison of the concentration and types of the proteins present in maxillofacial odontogenic cysts fluid

Odontogenic cysts concerning the concentration and type of proteins found in their fluid are different from each other in various cases. The main objective of this study was to compare and assess the concentration and the type of proteins found in odontogenic cysts fluid and to investigate their association with the recurrence of the cyst. In this descriptive and sectional study, cystic fluid of 24 patients referred to...

Author(s): Hamidreza Arabiun, Mohammad Mehdi Fani and Sahar Hosseini-Nejad