African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Characterization of Brucella species in Mexico by Bruce-Ladder polymerase chain reaction (PCR)

Aurea Itzel Morales-Estrada1, Joel Castillo-Salto1, Ahidé López-Merino1, María Rosario  Morales-García1,3, Juan Gabriel Valle-Valdez2 and Araceli Contreras-Rodríguez1*
1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México. 2Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México. 3CICATA Querétaro, Instituto Politécnico Nacional, México.
Email: [email protected]

  •  Accepted: 29 February 2012
  •  Published: 23 March 2012

Abstract

The typing of 153 Brucella strains isolated from different regions of Mexico was performed by multiplex PCR Bruce-Ladder. Results showed that microbiological typing and multiplex Bruce-ladder amplification were identical for all Brucella isolates tested. Because of its speed, Bruce-Ladder PCR is a useful method in the typing of Brucella species isolated from animal and humans.

 

Key words: Brucella, brucellosis, typing, Mexico, Bruce-ladder polymerase chain reaction (PCR).

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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