August 2013
Seroprevalence of pestivirus in small ruminants in Sudan
Pestiviruses are known to exist in ruminants worldwide; however in Sudan, sparse work has been published. This study is aimed at investigating the existence of pestivirus, particularly bovine viral diarrhea virus (BVDV) in small ruminants in Sudan through the detection of its antibodies in sheep and goat sera. A total of 424 sheep and 209 goat sera were collected from different localities in Sudan...
August 2013
Changes in the microbial population of pasteurized soursop juice treated with benzoate and lime during storage
The microbial quality of locally produced soursop juice and the effect of pasteurization, acidification and chemical preservative on the juice were investigated. Soursop juice samples were analyzed according to standard bacteriological and chemical methods. Microbiological quality of the treated juice (pasteurized at 60°C for 1 h, acidified with lime and addition of 0.05% sodium benzoate) was evaluated....
August 2013
Coliform bacterial estimation: A tool for assessing water quality of Manasbal Lake of Kashmir, Himalaya
Total coliform count is commonly used to assess contamination level of drinking and swimming waters, especially with pathogenic bacteria of intestinal origin. The common sources of coliforms in Kashmir Himalayan lakes include point source discharges (raw sewage, combined sewer overflows, effluents from wastewater treatment plants, industrial sources) and non-point source discharges (agriculture, forestry and...
August 2013
Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin
We develop an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) DNA fingerprinting technique for genotyping enterotoxigenic Escherichia coli(ETEC) in calves. Using the ERIC-PCR, we detected 66 ETEC strains in calves isolated from 11 farms. The ETEC strains could be grouped into 17 types, with type D predominant. The ERIC-PCR system is an efficient method for...
August 2013
Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan
Lallemantia royleana (Benth.) is an important folk medicine in Pakistan. This natural herb is used as folk remedy for number of ailments. This study was conducted to assess the antibacterial activity of four different organic extracts of L. royleana seeds against four bacterial strains (Escherichia coli, Enterobacter cloaceae, Pseudomonas aeruginosa andStaphylococcus aureus)...
August 2013
The applications of plant growth promoting rhizobacteria (PGPRs) as biofertilizers for cold area rice variety JP-5
The aim of present study was to evaluate the effect of bacterial inoculation on different growth parameters of rice variety JP 5. A total of 18 bacterial strains were isolated from roots and rhizosphere of rice. Three bacterial strains (Azospirillum brasilense strain R1,Azospirillum lipoferum strain RSWT1 and Pseudomonas strain Ky1) were used to inoculate rice variety JP 5 at two...
August 2013
Efficient production of l-asparaginase by marine Streptomyces sp. isolated from Bay of Bengal, India
Marine actinobacteria isolated from Bay of Bengal and evaluated for the l-asparaginase production capability. A total of 75 actinobacterial strains were isolated and screened for antibacterial activity. Among them, 10 actinobacterial strains showed significant antibacterial activity. Those strains were identified based on phenotypic and chemotaxonomic characteristics and screened for l-asparaginase...
August 2013
Adhesive properties and antibacterial susceptibility of Vibrio alginolyticus strains isolated from a Tunisian Ruditapes decussatus hatchery
Vibrio alginolyticus has been reported to cause several damages to reared bivalve molluscan larva. In the present work, we characterized fifty four phenotypic and genetic V. alginolyticus strains isolated from a Ruditapes decussatus hatchery in the National Institute of Marine Sciences and Technologies, Monastir, Tunisia, then we studied the antibiotics susceptibility and the...
August 2013
Epidemiology of mycobacterial infections in cattle in two districts of Western Tigray Zone, northern Ethiopia
A cross-sectional study was conducted from September, 2010 to July, 2011 on 1,066 cattle (484 dairy and 582 beef) in two districts of Western Tigray Zone, Northern Ethiopia, to investigate the epidemiology of mycobacterial infections in cattle and characterize its causative agents. Comparative intradermal tuberculin (CIDT) test, pathological and bacteriological examination, and molecular typing were...
August 2013
Comparison of physical characteristics and collection and elution performance of clinical swabs
Diagnostic sensitivity of a clinical test is a function of the number of organisms collected and eluted from the swab head that is attributed to the type of fibers and their physical characteristics. To relate these properties, water and protein absorption of nylon flocked, rayon flocked, and HydraFlock® and macrofoam swabs manufactured by Puritan Medical Products, and nylon flocked swab made by Copan...
August 2013
Molecular diagnosis of enterovirus infections in Chungnam Korea in 2010
The epidemiological data of enteroviruses isolated in Chungnam, Korea since 2005 has been reported, and this current study extends these observations in 2010. During this period, 178 of the total 920 samples (19.3%) were positive for enterovirus, and the types were identified using nucleotide sequence analysis in VP1. The temporal distribution was similar to previously reported data, however, coxsackievirus A6 and...
August 2013
Prevalence of Listeria species in raw hamburger meatballs and chicken burgers in eastern Turkey
The aim of this study was to determine the prevalence of Listeria spp. in raw hamburger meatballs and chicken burgers obtained from different fast-food and markets in Elazig and Malatya Provinces in the east of Turkey during 2010 and 2011. A total of 71 samples, consisting of 35 samples of raw hamburger meatballs and 36 samples of chicken burgerswere...
August 2013
Prevalence and antibiotic resistance of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey
The present study includes 50 Klebsiella oxytoca, 45 Escherichia coli and 13 Klebsiella pneumoniae isolated from raw meat (chicken drumsticks and minced meat), raw milk, white cheese and ice cream samples and was undertaken to determine the prevalence of extended-spectrum β-lactamase (ESBL)-producing isolates and antibiotic resistance. The double-disk synergy test was used to...
August 2013
Directly accessing the diversity of bacterial type I polyketide synthase gene in Chinese soil and seawater
Many bacteria are known to produce a wide range of biomedically important secondary metabolites synthesized by type I polyketide synthases (PKSs). These enzymes own a modular structure that could be used for combinatorial biosynthesis to yield novel drug candidates. To directly access PKS gene diversity of soil and seawater in China, a set of degenerate oligonucleotide primers were designed to amplify the...
August 2013
Antagonistic aptitude and antiproliferative properties on tumor cells of fungal endophytes from the Astroni Nature Reserve, Italy
As a yet thoroughly explored component of biodiversity, endophytic fungi are stimulating a huge research activity worldwide concerning their occurrence, biocenotic role, and opportunities for exploitation in biotechnologies. This paper presents the results of an investigation on fungal endophytes from 28 species of trees and shrubs thriving at the Astroni Nature Reserve near Naples, Italy. One hundred and...
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