July 2014
Plant lectins as alternative tools against bacterial biofilms
Plant lectins has the ability to interfere in crucial biofilm formation aspects, playing a significant role in evaluation of patients at high and low risk of disease development. However, there is limited studies on the physiological role of lectins on bacteria living in biofilms like caries process. Thus, we aimed to provide a view of lectins biotechnological potential against bacterial biofilms development. Biofilm is...
July 2014
Production of alkaline protease from a haloalkaliphilic soil thermoactinomycete and its application in feather fibril disintegration
Proteases have found a wide application in several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. In the present study, a bacterial isolate, Thermoactinomyces sp. RS1, isolated from soil sample was taken for enzyme production by submerged fermentation technology. The classical...
July 2014
Antimicrobial and antioxidant activities of red onion, garlic and leek in sausage
This study was designed to evaluate antimicrobial and antioxidant effect of ethanolic and aqueous extracts and essential oils of red onion, garlic and leek against Escherichia coli O157:H7, Staphylococcus aureus, A.F., 4, Salmonella typhimurium, A.F., 3, Aspergillus niger, H.U.B., 1, Aspergillus ochrecies, H.U.B.,12 and Fusarium oxysporum, H.U.B., 3 in sausage. The susceptibility of these isolates toward the extracts of...
July 2014
Microbiological studies on resistance patterns of antimicrobial agents among Gram negative respiratory tract pathogens
Respiratory tract infections (RTIs) are the most frequently-occurring infections of all human diseases and have been frequently documented. This study investigated the antimicrobial resistance patterns among Gram negative respiratory tract isolates. A total of 309 non replicate Gram negative respiratory tract isolates were collected and identified. Molecular mechanisms of antimicrobial resistance pattern were...
July 2014
Disease reaction studies of maize (Zea mays L.) against turcicum leaf blight involving indigenously identified cytosterile source
Among biotic stresses affecting maize, the turcicum leaf blight caused by Exserohilum turcicum is one of the most important diseases in India. Disease reaction studies against turcicum leaf blight were done with two crosses viz., 15C (A) x I-318 (R) and I-401(A) x I-318(R) for all six generations with P1, P2 and F1 having 30 plants each and F2 (300 plants), BC1 (180 plants) and BC2 (180 plants). Analysis of variance of...
July 2014
Detection and characterization of bacteriophages attacking dairy Streptococcus thermophilus starter cultures
Sixty (60) strains of Streptococcus thermophilus isolated from dairy traditional Egyption yoghurt samples were characterized. The yoghurt samples were collected from the local markets in Egypt. The results showed that few phages can attack S. thermophilus strains isolated from traditional Egyptian yoghurt (Zabady) samples, only three temperate phages were isolated. The isolated phages attacking samples were ...
July 2014
Evaluation of Pseudomonas isolates from wheat for some important plant growth promoting traits
Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms. The use of PGPR is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present study, isolation and characterization of PGPR from the rhizospheric soil of wheat was done for the enhancement...
July 2014
Prevalence of antimicrobial resistant Aeromonas in chicken and fish washings
This study was undertaken to assess prevalence of virulent and antibiotic-resistant Aeromonas species in chicken meat and fresh water fish washings procured from local market. Isolation was done on three selective agar viz. Aeromonas isolation media, ampicillin dextrin agar and Aeromonas starch DNA agar. Presumptive colonies were directly screened by multiplex polymerase chain reaction (PCR) targeting genus specific 16S...
July 2014
Phosphate solubilization and phytohormone production by endophytic and rhizosphere Trichoderma isolates of guanandi (Calophyllum brasiliense Cambess)
This work aimed to isolate and evaluate the phosphate solubilization and phytohormone production abilities of endophytic and rhizosphere fungi belonging to the genus Trichoderma isolated from guanandi (Calophyllum brasiliense Cambess). From the guanandi collected from the field, 12 isolates obtained were grown in potato dextrose broth and supplemented with the phosphate sources, to test their capacity to solubilize...
July 2014
Lipopolysaccharide isolated from Rhizobium leguminosarum strain P.SOM induces resistance in pea roots against Orobanche crenata
Our previous studies showed that living and heat-killed cells of the Rhizobium leguminosarum strain P.SOM induce in pea roots systemic resistance to infection by Orobanche crenata. To better understand the mechanisms of induced resistance, we focused on identifying the inducing agent. Since heat stable bacterial surface carbohydrates such as exopolysaccharides (EPS) and lipopolysaccharides (LPS) are essential for...
July 2014
Detection and biovar discrimination of Ureaplasma urealyticum colonization in preterm neonates under ventilation and correlation with bronchopulmonary dysplasia
Despite numerous studies, controversy regarding the association between Ureaplasma urealyticum colonization of premature neonates and bronchopulmonary dysplasia (BPD) still exists. The aim of the present study was to determine the colonization rate of preterm ventilated neonates with the two U. urealyticum biovars, parvo and T960, and to assess the correlation between colonization and the development of BPD. A...
July 2014
Assessment of the biological activity of kefir grains by biospeckle laser technique
This work presents the biospeckle laser technique as a potential tool to analyse the kefir grains activity. In the present work, the kefir grains biological activity was measured from quantitative measurements by means of their speckle activity. The aim was to show that the biospeckle laser is a potential methodology to assess kefir grains viability, monitoring the kefir grains during the beverage production. The...
July 2014
Methicillin-resistant Staphylococcus aureus (MRSA) colonization rate among ruminant animals slaughtered for human consumption and contact persons in Maiduguri, Nigeria
This study determined the methicillin-resistant Staphylococcus aureus (MRSA) colonization rate among ruminant animals slaughtered for human consumption and contact persons. Nasal and milk product samples were collected from the main abbatoir in Maiduguri and analyzed using standard bacteriological procedures. A total of 510 samples were analyzed, 87 (17.1%) S. aureus isolates were identified, 33 (34.6%) MRSA and 54...
July 2014
A survey of the microflora of the outdoor air environment of Keffi Metropolis, Nasarawa State, Nigeria
The microbiological quality of the air environment of Keffi metropolis was assessed by determining the concentrations and composition of bacteria and fungi present in the outdoor air. Air samples were collected from ten different locations of the metropolis by the plate sedimentation methods which involved exposing media-filled Petri plates to the air for 30 min. Trypticase soy agar (TSA) was used for the enumeration of...
July 2014
Enhanced immune responses in mice to combined immunization with Mycobacterium tuberculosis Ag85A and DDA/MPL
The tuberculosis (TB) Bacillus Calmette-Guerin (BCG) vaccine, which is based on Mycobacterium bovis, has variable protective efficacy in humans; therefore, safe and effective vaccines are urgently required to prevent this disease. The antigen 85A from Mycobacterium tuberculosis (M. tuberculosis) is a prime target for immunity in the early phase of tuberculosis in various animal models. The purpose of this study was to...
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