African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Enhanced immune responses in mice to combined immunization with Mycobacterium tuberculosis Ag85A and DDA/MPL

Dan Zhao
  • Dan Zhao
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Weijun Tan
  • Weijun Tan
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Zhengzhong Xu
  • Zhengzhong Xu
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Xiang Chen
  • Xiang Chen
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Shuyang Ye
  • Shuyang Ye
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Kai Lian
  • Kai Lian
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Zhiming Pan
  • Zhiming Pan
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Yuelan Yin*
  • Yuelan Yin*
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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Xinan Jiao*
  • Xinan Jiao*
  • Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China
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  •  Received: 28 March 2014
  •  Accepted: 13 June 2014
  •  Published: 02 July 2014

Abstract

The tuberculosis (TB) Bacillus Calmette-Guerin (BCG) vaccine, which is based on Mycobacterium bovis, has variable protective efficacy in humans; therefore, safe and effective vaccines are urgently required to prevent this disease. The antigen 85A from Mycobacterium tuberculosis (M. tuberculosis) is a prime target for immunity in the early phase of tuberculosis in various animal models. The purpose of this study was to confirm whether Ag85A could elicit protective immune responses in mice. Dimethyldioctadecylammonium (DDA), an adjuvant with Th1-promoting activities and monophosphoryl lipid A (MPL), an immunostimulatory component that has strong adjuvant activity for both cellular and humoral immune responses, has been used as the adjuvant to study the biological and immunological characteristics of tuberculosis proteins in the researches previously. The gene coding Ag85A (fbpA), was cloned into a pET-30a(+) prokaryotic expression vector, and the induced amino acid sequence corresponds to a 33 kDa protein, which was confirmed by mass spectrometry and western blots. Mice were subcutaneously immunized with Ag85A protein emulsified with DDA and MPL and the results showed that the 85A antigen, when combined with these adjuvants, elicited strong Ag85A-specific T-cell responses and humoral responses as compared with vaccination with BCG. Based on the fact that this vaccine combination induced strong antigen-specific immune responses, it is a prime candidate as a component of a future TB vaccine.

Key words: Tuberculosis, Ag85A, prokaryotic expression, cellular immune response, humoral response.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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