June 2010
Isolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Five spore-forming bacteria producer of lactic acid were isolated from soils sampled in the vicinity of poultry farms in Burkina Faso. All isolates were Gram-positive, motile, mesophilic, facultative anaerobic, catalase positive rods, and with L(+) lactic acid production. The isolates have been characterized and identified by a polyphasic approach, combining various phenotypic and genetic characteristics. The...
June 2010
Genetic diversity of Fusarium oxysporum f. sp. cubense isolates from Malaysia
Genetic diversity of Fusarium oxysporum f. sp. cubense (FOC) isolates were analyzed using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), random amplified microsatellites (RAMS) and restriction fragment length polymorphism of Intergenic Spacer (RFLP-IGS). The three molecular techniques showed intraspecific variations and the banding patterns generated using each technique...
June 2010
Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis
Leishmaniasis is a common and significant parasitic disease among the Middle East and Mediterranean countries. Cutaneous and visceral forms of leishmaniasis are seen in Turkey. Direct microscopic examination of Giemsa-stained smears and inoculation into a culture medium are gold standards in diagnosis. In the present study, three diffferent culture media (pepton-yeast extract, brain heart and nutrient broth)...
June 2010
Growth control of listeria monocytogenes in experimental cheese samples by lactobacillus casei RN 78 and its bacteriocin
Anti-listerial effect of freeze dried fractions of partially purified bacteriocin (Fd-PPL) and the producer strain Lactobacillus casei RN 78 strain was evaluated in experimental cheese samples during storage at two different temperatures (4 and 35ËšC). With the addition of 6400 AU/g of Fd-PPL fractions to the cheese samples of the initial concentration of 4.81 +/- 0.06 log...
June 2010
Biofilm formation potential of oral streptococci in related to some carbohydrate substrates
In this study, the effect of sugars type (glucose, sucrose, fructose and the mixture of glucose and fructose) and best substrate concentration are investigated on biofilm accumulation. Then, the role of this substrate examined on gtfB expression level by real time RT PCR. A total of 40 Streptococci strains isolated from dental caries and plaque of patients. The ability of them to form biofilm measured in the...
June 2010
Production and characterization of protease enzyme from Bacillus laterosporus
Production and partial purification of protease enzyme by Bacillus laterosporuswas the aim of this study. B. laterosporus was allowed to grow in shake flask broth culture for purpose of inducing protease enzyme. The protease enzyme was purified by ammonium sulfate precipitation followed by dialysis and further concentrated by Amicon tubes. After concentration, the protein was subjected to 12%...
June 2010
Genetic characterization of osmotolerant fermentative saccharomyces yeasts from Tanzania suitable for industrial very high gravity fermentation
Naturally occurring yeasts were sought from diverse Tanzanian environments and screened for industrial application. The yeasts were isolated from environments such as traditional brews and wines from various parts of Tanzania. In this regard, a total of thirty yeast isolates were screened for their suitability in Industrial Very High Gravity Fermentation (VHGF). Five of these isolates were able to grow and...
June 2010
Nasal colonization of symptomatic pneumonia patients in university of Benin teaching hospital, Benin City, Nigeria by multiple antibiotic resistant Staphylococcus aureus
A study of the antibiotic resistance pattern of Staphylococcus aureus from the nasal cavities of patients with pneumonia attending the University of Benin Teaching Hospital, Benin City, Nigeria was conducted. The survey revealed S. aureus to be the most predominant cause of Staphylococcal pneumonia with an occurrence rate of 38.5%. Other microorganisms encountered includedStaphylococcus...
June 2010
In vitro activity of Thaumatococcus daniellii and Megaphrynium macrostachyum against spoilage fungi of white bread and ‘Eba’, an indigenous staple food in southern Nigeria
Fresh white bread and ‘eba’ (a carbohydrate; > 80% starch, indigenous staple food in southern Nigeria) were purchased from traders in Lagos, Nigeria and stored under aseptic conditions in the laboratory at room temperature (27 ± 2°C) (11 h of darkness and 15 h of daylight) for duration of 144 h, during which samples were analyzed at 48 h intervals for fungal population using the...
June 2010
Cloning and phylogenetic analysis of gD gene of human herpes simplex virus type 2 in Iranian isolates
Herpes simplex virus type 2 (HSV-2) is a member of herpesviridae family and subfamily of alphaherpesvirinae. HSV-2 infection often causes genital herpes in women and men, abortion, infant herpes and non-infectious meningitides. Glycoprotein D and B respectively, are coded by gD and gB gene. They are the adhesion of HSV-2 attached to the surface of the epithelial cells and are...
June 2010
Investigation of Extended Spectrum Beta Lactamase Production of Bacteria by Direct Urine Inoculation
Extended spectrum beta lactamase (ESBL) production of bacteria was investigated by direct urine inoculation method in urine samples considered to be infected with Enterobactericeae. Urine samples were aliquoted to two sterile containers. One container was used for microbiological examination and the other one was used for dipstick analyses. ESBL phenotype (both in the direct test and in the standard...
June 2010
Protective effects of sucralfate on anti-H. pylori VacA IgY in vivo and in vitro
This study aimed to evaluate the protective effects of sucralfate on immunoglobulin yolk (IgY) and to provide a theory basis for preparing IgY oral preparations which can tolerate acid and pepsin. Engineering bacteria DH5α-vacA-pQE30 were largely induced to express recombinant vacuolate cytotoxin antigen (VacA). VacA antigen was inoculated in Lohman hen and IgY was purified. Different concentrations of...
June 2010
Antifungal activities of neem (Azadirachta indica) seed kernel extracts on postharvest diseases in fruits
To learn the antifungal effects of neem seed kernel extract (NE) on the post harvest diseases, pathogens of Monilinia fructicola, Penicillium expansum, Trichothecium roseum and Alternaria alternate isolated from the infected fruit were treated with NE in vitro. Results showed that growth of the four pathogens could be significantly (P < 0.05) reduced by NE. The diseases in fruit of...
June 2010
Comparison of cell growth and ethanol productivity on different pretreatment of rice straw hemicellulose hydrolysate by using Candida shehatae CICC 1766
In this work, the cell growth and ethanol productivity was tested forlignocellulosic biomass sugars of overliming-detoxified and NaOH-neutralized rice straw acid hydrolysate by using Candida shehatae CICC 1766. When the acetic acid reached 3 g/L in defined xylose medium, the final ethanol concentration was 33% of that in the medium without acetic acid addition. C....
June 2010
Prevalence of bacterial infection responsible for bovine mastitis
Mastitis continues to be the most economically important disease of dairy cattle, and current data on mastitis is even less readily available. To investigate into the prevalence of the pathogens responsible for bovine mastitis, 100 raw milk samples were obtained from the dairy cows with clinical or sub-clinical bovine mastitis in 5 farms in Jiangsu Province, China. All the samples weresubmitted to...
June 2010
Seroprevalence of toxoplasmosis in high school girls in Bushehr city, South west of Iran, 2009
Toxoplasmosis is a parasitic disease prevalent all over the world and it’s causal agent is Toxoplasma gondii. Toxoplasmosis is very important in pregnant women. Seroepidemiological survey of toxoplasmosis in young girls before marriage for identifying non immune girls could be used to prevent congenital toxoplasmosis. A seroepidemiological study of toxoplasma IgG-antibody in 516 high...
June 2010
Effect of Mentha longifolia L. ssp longifolia essential oil on the morphology of four pathogenic bacteria visualized by atomic force microscopy
The chemical composition and the antibacterial activity of the essential oil ofMentha longifolia L. ssp longifolia are being investigated in the present work. The minimal inhibitory concentration (MIC) of this oil against four gram+ and gram- reference bacteria including Salmonella typhimurium LT2, Escherichia coli ATCC 35218, Microccus luteus NCIMB 8166...
June 2010
Detection of active E. coli after irradiation by pulsed UV light using a Qβ phage
The infectivity rates of Qβ phage is used as a bio-indicator of the physiological state of host cells and for the detection of active Escherichia coli irradiated by an increasing number of pulsed ultra-violet (UV) light. Indeed, the combination of a conventional method used to measure colony-forming ability of tested bacteria after exposure to an increase number of pulsed UV light,...
June 2010
Beneficial effects of Bacillus subtilis on field-grown tomato in Burundi: Reduction of local Fusarium disease and growth promotion
The biocontrol potential of Bacillus subtilis S499 was evaluated on tomato in open field sites in low altitude area of the plain of Imbo in Burundi. This strain was tested in order to reduce the impact of an important fungal disease giving rise to large losses in local plantings. The causing pathogen was isolated from diseased leaves at different locations in the fields and identified...
June 2010
Comparison of cell surface hydrophobicity and biofilm formation among ESBL-and non–ESBL-producing Pseudomonas aeruginosa clinical isolates
Pseudomonas aeruginosa is a major cause of nosocomial infections. Recently multidrug resistance and extended-spectrum ß-lactamase (ESBL)-producing P. aeruginosa isolates are emerging worldwide. These isolates are reported to be more virulent than the non multidrug resistance and non ESBL producing isolates. In order to find a correlation between ESBL production and virulence, we tested...
June 2010
Purification and characterization of intracellular nitrilases from rhodococcus sp. - potential role of periplasmic nitrilase
Two bacterial species Rhodococcus rhodochrous and Rhodococcus fascianswere evaluated for the production of nitrilase enzyme (EC 3.5.5.2) from benzonitrile. This is the first time report on comparative analyses of intracellular nitrilases in two different Rhodococcus species which showed the involvement of periplasmic nitrilases towards nitrile degradation rather than...
June 2010
Phenolic profiles, antimicrobial and antioxidant activity of the various extracts of Crocus species in Anatolia
The phenolic profile and quantitative composition of methanol extracts of Crocus baytopiorum which was endemic species in Denizli, Turkey was detected by high performance liquid chromatography (HPLC-DAD). The HPLC analysis of phenolic compounds in methanol extract of C. baytopiorum showed that p-Coumaric acid, apigenin-glucoside, rosmarinic acid, quercetin and kampferol were present. Also,...
June 2010
Determination of acrylamide concentration in microbe free processed food products available in Indian market using GC-MS
n present scenario acrylamide concentration in processed food products have become a very serious health issue. The world health organization (WHO) and the scientific committee for food (SCF) of the European Union also confirmed this concern. In laboratory scale, it was found that acrylamide causes tumors in animals. It is even present in processed food free of microbes indicating the absence of microbial...
June 2010
Preliminary study on the degradation of seven organophosphorus pesticides in bovine milk during lactic acid fermentation or heat treatment
Bovine milk was spiked with seven organophosphorus pesticides, denthion, dimethoate, malathion, methyl parathion, monocrotophos, phorate and trichlorphon, and subjected to heat treatment at 63, 80 and 100°C or lactic acid fermentation at 42°C with commercial directed vat set (DVS) starters to investigate the degradation kinetics of the pesticides in bovine milk during heat treatment and yoghurt...
June 2010
Protein expression and phylogenetic analysis of thymidine kinase gene of avian infectious laryngotracheitis virus in Iran
Avian infectious laryngotracheitis (ILT) is a severe clinical respiratory disease of chickens and causes the clinical symptoms of difficulty in breathing and bloody coughing and as if involves laying hens affect the egg production. In order to cloning of the coding region of TK gene of ILT virus , PCR product of the open reading frame of the gene from DNA extracted of infectious tissue of involved...
June 2010
Multiplex polymerase chain reaction (PCR) assays for the detection of Enterobacteriaceae in clinical samples
The accurate and rapid identification of bacteria in the enteric tract is necessary for early treatment. In this study, we describeD a novel system which consists of a multiplex polymerase chain reaction (PCR) to simultaneously identify a group of six Enterobacteriaceae members including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Citrobacter spp., Enterobacter...
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