Abstract

Extended spectrum beta lactamase (ESBL) production of bacteria was investigated by direct urine inoculation method in urine samples considered to be infected with Enterobactericeae. Urine samples were aliquoted to two sterile containers. One container was used for microbiological examination and the other one was used for dipstick analyses. ESBL phenotype (both in the direct test and in the standard test) was determined by disc diffusion method. 146 urine samples were tested for phenotypic screening and confirmation tests by direct inoculation method. 110 of them were found to be positive for leukocyte esterase (LE) and nitrite (NIT) with dipstick analyses. 36 of them were found to be positive for LE only with dipstick analyses and containing gram negative bacilles with gram staining. Standard ESBL testing was also performed in parallel. ESBL production was detected in 38 bacteria with direct inoculation method and also confirmed with standart methods. Results of the two tests were in concordance. In conclusion, ESBL production of bacteria could be detected earlier with direct inoculation method compared to standard inoculation method. Use of this method could expedite the initiation of appropriate antimicrobial therapy in hospitals known to have higher rates of ESBL production. 

Key words: Urinary tract infections, antimicrobial resistance, extended spectrum beta lactamase.