African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 4 June, 2013; 7(23)

June 2013

Effects of the different nitrogen, phosphorus and carbon source on the growth and glycogen reserves in Synechocystis and Anabaena

The effects of different nutrient sources (nitrogen, phosphorus and carbon) on the growth status and glycogen reserves in Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 were studied. The two cyanobacteria grew well on suitable nutrient sources, for example, nitrate (sodium nitrate), phosphate (two potassium hydrogen phosphate) or inorganic (sodium carbonate, dicarbonate) and...

Author(s): Xing Wang, and Lusan Liu,

June 2013

Development and characterization of a symbiotic cheese added with Saccharomyces boulardii and inulin

The aim of this work was to develop and characterize a functional food (fresh cheese) by incorporation of a probiotic microorganism (Sacharomyces boulardii) and a prebiotic agent (inulin), both encapsulated with sodium alginate and cactus mucilage. The microorganism viability incorporated in the fresh cheese in free and encapsulated form was evaluated at the beginning and the end of the cheese shelf life. The...

Author(s): Rafael Zamora-Vega, José Luis Montañez-Soto, José Venegas-González, Aurea Bernardino-Nicanor, Leopoldo González Cruz and Héctor Eduardo Martínez-Flores

June 2013

Acid phosphatase activity of the pantropical fungus Scleroderma sinnamariense in pure culture

Ectomycorrhizal fungi have been shown to improve on the phosphorus nutrition of their plant partners and acid phosphatase activities are thought to be important in the uptake process. The effect of nitrogen source and presence/absence of phosphorus in the experimental medium on acid phosphatase activity of Scleroderma sinnamariense, an ectomycorrhizal fungus associated with Gnetum Cameroon, was...

Author(s): Eneke Esoeyang Tambe Bechem

June 2013

Isolation of DNA from saltern soils collected in Taiwan and whole-genome amplification of minute amounts of DNA for construction of metagenomic libraries

Environmental DNAs from 21 samples of saltern soil in Taiwan were isolated by using the SDS-lysis method, resulting in yields ranging from 0.03 ng to 8.06 µg per gram of soil. However, sample 143 collected from saltern soil near a crystallizer had a low yield of 1.2 ng per gram of soil. Comparative analyses of the sequence data of representative clones with other 16S rRNA samples indicated that not all clones for...

Author(s): Shie-Jea Lin, Hsuen-Chun Liao, Tzu-Jun Liu and Wen-Shen Chu

June 2013

Antiserum prodction and reverse transcription polymeraes chain reaction (RT-PCR) for detection of bean leaf roll virus

Bean leaf roll virus (BLRV) is a recently isolated virus from Vicia fabae beans which was collected from eight different locations at Ismailia governorate, Egypt. This virus is belonging to the genus luteovirus and resembles its members in mode of transmission, symptoms and morphological appearance. In this study, BLRV was identified using multi techniques, direct enzyme linked...

Author(s): El-beshehy, E. K. F., and Azza, G. Farag,

June 2013

Analysis of 16S ribosomal RNA gene segments for the diagnosis of Gram negative pathogenic bacteria isolated from urinary tract infections

Bacterial 16S ribosomal RNA (rRNA) genes contain nine “hyper-variable regions” (V1–V9) that demonstrate considerable sequence diversity among different bacteria. Species-specific sequences within a given hyper-variable region constitute useful targets for diagnostic assays and other scientific investigations. No single region can differentiate among all bacteria; therefore, systematic studies that...

Author(s): Abdelaziz Elgaml, Ramadan Hassan, Rasha Barwa, Shadi Shokralla, and Wael El-Naggar

June 2013

Effectiveness of menthol and folium menthae piperitae against Acinetobacter baumannii

Acinetobacter baumannii is a common Gram negative nosocomial pathogen. Although new agents have been developed against Gram positive microorganisms, there are few new antibacterial agents against Gram negative bacteria. In this environment, herbal medicine has become a flicker of hope. The purpose of this study was to investigate the antibacterial activity of menthol and folium menthae piperitae extract either...

Author(s): Ertugrul Guclu, Hayriye Genc,  Mustafa Zengin and Oguz  Karabay

June 2013

Uptake of some heavy metals by metal resistant Enterobacter sp. isolate from Egypt

The bacteria displaying a high growth rate were isolated from polluted industrial waste water. The bacteria Enterobacter sp. had variable resistant to heavy metals such as Cd, Cu, Co, Zn and Pb. Out of which the minimal inhibitory concentrations were 0.4, 1.0, 1.0, 2.0 and 3.0 mM, respectively. The potent bacterium had optimal biosorption capacity raised according to the metal, incubation...

Author(s): Abd El-Raheem R. El-Shanshoury, Sobhy E. Elsilk, and Perihan S. Ateya

June 2013

Initiation of fruiting body development of the medicinal mushroom Phellinus linteus from Cambodia

The purpose of this study was to determine the mating type system and initiation of fruiting body development of a medicinal mushroom Phellinus linteus from Cambodia.  Theevidence of fungal crossing analysis revealed that Cambodian P. linteus was a heterothallic basidiomycete and had a bipolar mating system. Clamp connection and peg cells formed on the dikaryons were possibly regulated...

Author(s): Prayook Srivilai, Ananyaphon Prommetta and Preecha Prathapa

June 2013

Phenotypic and molecular characterization of plant growth promoting Rhizobacteria isolated from the rhizosphere of wheat (Triticum durum Desf.) in Algeria

Several strains were isolated from rhizosphere of wheat in Eastern Algeria. All the 10strains (Azo4, Azo5, Azo6, Azo7, Azo8, Azo10, S1, S2, S3 and S9) were identified; based also on the characteristics which are not only morphological, characteristics such as shape but physiological ones: optimal temperature, pH optimum and NaCl tolerance. These isolates were screened in vitro for their growth promoting traits...

Author(s): Benmati Mahbouba, Le Roux Christine, Belbekri Nadir, Ykhlef Nadia, and Djekoun Abdelhamid,

June 2013

Screening of beneficial properties of rhizobacteria isolated from Saffron (Crocus sativus L) rhizosphere

Plant growth promoting rhizobacterias (PGPRs) are free living soil bacteria that colonize root surfaces and have the capacity to enhance plant growth directly or indirectly. A total of 23 bacterial strains were isolated from saffron rhizoshere soil during the flowering stage of corms. All these isolates were screened for their plant growth promoting traits like production of IAA, phosphate solubilisation activity and...

Author(s): Javid A. Parray, Azra N. Kamili, Zaffar A. Reshi, Rehana Hamid and Raies A. Qadri

June 2013

Evaluation of postmortem bacterial culture results: A retrospective study

Postmortem blood, cerebrospinal fluid (CSF) and various clinical samples are sent to the medical microbiology laboratory for bacteriological examination in order to determine the cause of death. This study aimed to investigate the results of postmortem cultures sent to our laboratory from different clinics between January 2005 and August 2011. The culture results were retrospectively analyzed from the laboratory...

Author(s): Melek Demir, Nural Cevahir, Osman Acar, Ergun Mete, ÇaÄŸrı Ergin and Ä°lknur Kaleli  

June 2013

Face to face with Actinobacillus pleuropneumoniae: Landscape of the distribution of clinical isolates in Southeastern Brazil

Pleuropneumonia caused by Actinobacillus pleuropneumoniae is one of the most devastating diseases in the scenario of swine global production, in which Brazil occupies a prominent place. A. pleuropneumoniae have been shown to be difficult to type by the existing methods given its existence in 15 different serotypes. The goals of this study were to establish an identification experimental strategy...

Author(s): Ciro César Rossi, Alexandre Magno Vicente, Walter Vieira Guimarães, Elza Fernandes de Araújo, Marisa Vieira de Queiroz and Denise Mara Soares Bazzolli

June 2013

Characterization of a thermostable polysaccharide bioflocculant produced by Virgibacillus species isolated from Algoa bay

We evaluated the bioflocculant production by Virgibacillus species isolated from a marine environment in the Eastern Cape Province of South Africa. Culture conditions for optimal production of the bioflocculant included: glucose as sole carbon source (flocculating activity 91.8%); complex nitrogen source (urea + yeast extract + (NH4)2SO4); inoculum size of 2% (v/v); initial pH 10; and calcium as mediating...

Author(s): Cosa Sekelwa, Ugbenyen M. Anthony, Mabinya L. Vuyani and Okoh I. Anthony

June 2013

The antibacterial biofilm activity of metal-doped mullite ceramics against pathogenic bacteria

Bacterial biofilms are densely packed microorganisms surrounded by secreted polymers responsible for many chronic infectious diseases as well as for contamination of clinical and industrial environments. The aim of this study was to assess the potential antimicrobial activity of zinc-, copper- and silver-doped mullite ceramics disks against biofilm producing clinical isolates of Staphylococcus...

Author(s): Suzan A. Matar, Eman M. Mahmoud and Hamdallah A. Hodali

June 2013

Anti-candidal activity and effect on relative cell surface hydrophobicity of Pongamia pinnata

Pongamia pinnata is used in Sri Lankan traditional medicinal systems to cure diseases including microbial infections. Different parts of this plant were investigated for in vitro anti-candidal activity. Extracts of bark, roots and leaves of P. pinnata were examined for their activity on different Candida spp. using well diffusion assay. The extract with best activity was evaluated for...

Author(s): Dinusha K. Kanatiwela, Charmalie L. Abayasekara, Nimal K.B. Adikaram, Nipuna B. Parahitiyawa, Dammantha M. Senanayake and Gehan J. Panagoda

June 2013

Zoonotic parasitic diseases in Ilam Province, Western Iran

Zoonotic parasitic diseases are a significant and widespread public health threat, globally. This study aimed to investigate the prevalence of zoonotic parasites in slaughtered animals at industrial abattoir in Ilam Province, Western Iran. In the present descriptive and retrospective study, all records of abattoir were analyzed....

Author(s): Abdi J, Karimi S. H. and Naserifar R.

June 2013

Isolation and identification of Leuconostoc mesenteroides producing bacteriocin isolated from Algerian raw camel milk

Eighty three strains of Leuconostoc mesenteroides were isolated from 12 samples of raw camel milk collected from different Algerian zones. Based on morphological, biochemical and physiological characters tests, 36.52% strains were identified as L. mesenteroidesspp. mesenteroides, 30.71% as L. mesenteroides spp. dextranicum and 3.32% as L....

Author(s): Chentouf Hanane Fatma and Zineb Benmechernene

June 2013

Strain improvement of Rhizopus oryzae for over-production of lactic acid by random mutations

Rhizopus oryzae mutant strain LA-UN-1 was isolated from the lactic acid-producing strain NRRL 395 by mutagenesis with both ultraviolet (UV) irradiation and nitrosoguanidine (NTG) treatment with the aim of selecting mutant in pellet form. One of the mutants in flask culture easily formed the pellet morphology at a diameter between 1.1 and 1.3 mm. In this case, the lactic acid concentration...

Author(s): Longfei Yin, Qicheng Ruan and Yongqian Fu

June 2013

Effect of Pediococcus parvulus and Candida parapsilosis on growth and survival of tilapia, Oreochromis niloticus and Oreochromis sp.

This study examined the effect of probiotic lactic acid bacteria (LAB) and yeast on growth and survival of tilapias, Oreochromis niloticus and Oreochromis sp. LAB and yeast were included in the diet. Microorganisms were identified at molecular level. The first bioassay (120 days, weight = 0.18 ± 0.03 g) was done with Oreochromis sp. with a control group, and animals fed with the...

Author(s): Antonio Luna-González, Daniel Quiñónez-Zúñiga, Jesús A. Fierro-Coronado, Héctor A. González-Ocampo, Ángel I. Campa-Córdova, Ma. Del Carmen Flores-Miranda and Viridiana Peraza-Gómez