African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Effects of the different nitrogen, phosphorus and carbon source on the growth and glycogen reserves in Synechocystis and Anabaena

Xing Wang1,2 and Lusan Liu1,2*
1State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. 2State Environmental Protection Key Laboratory of Estuarine and Coastal Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Email: [email protected]

  •  Accepted: 20 May 2013
  •  Published: 04 June 2013


The effects of different nutrient sources (nitrogen, phosphorus and carbon) on the growth status and glycogen reserves in Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 were studied. The two cyanobacteria grew well on suitable nutrient sources, for example, nitrate (sodium nitrate), phosphate (two potassium hydrogen phosphate) or inorganic (sodium carbonate, dicarbonate) and organic (sodium acetate, glucose) carbon. On the contrary, the growth rate decreased markedly when grown on ill-suited nutrient sources, for example, ammonium (ammonium chloride), organic nitrogen (urea), pyrophosphate (sodium pyrophosphate) and organophosphate (D-Glucose,6-(dihydrogen phosphate) sodium salt (1:2), adenosine-triphosphate). The yield of phycocyanin and chlorophyll a was higher when grown on suitable than ill-suited nutrient sources, whereas, the activities of superoxide dismutase (SOD) and peroxidase (POD) were higher on ill-suited than suitable nutrient sources, and the glycogen reserves presented the same variation tendences as peroxidase. These results indicate that the nutrient sources used in this study were involved in regulation of (1) the contents of pigments and glycogen, (2) energy and electron transport efficiencies of photosynthesis, and (3) activity level of SOD and POD in Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120.


Key words: Nitrogen, phosphoruscarbongrowth, glycogen reserves.