African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Antioxidant and antinociceptive effect of the hydroethanolic extract and fractions of the bark of Bowdichia virgilioides in orofacial pain

Andrea Yu Kwan Villar Shan
  • Andrea Yu Kwan Villar Shan
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Elis Cristiane Valença de Almeida
  • Elis Cristiane Valença de Almeida
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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André Luiz Lima Menezes dos Santos
  • André Luiz Lima Menezes dos Santos
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Amanda do Carmo Bion de Lima
  • Amanda do Carmo Bion de Lima
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Clisiane Carla de Souza Santos
  • Clisiane Carla de Souza Santos
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Marília Trindade de Santana Souza
  • Marília Trindade de Santana Souza
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Nicole Prata Damascena
  • Nicole Prata Damascena
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Silvan Silva de Araújo
  • Silvan Silva de Araújo
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Jymmys Lopes dos Santos
  • Jymmys Lopes dos Santos
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Monica Silveira Paixão
  • Monica Silveira Paixão
  • Department of Odonthology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil.
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Anderson Carlos Marçal
  • Anderson Carlos Marçal
  • Laboratory of Research in Intracellular Signaling, Department of Morphology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil.
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Lucindo José Quintans Júnior
  • Lucindo José Quintans Júnior
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Brancilene Santos de Araujo
  • Brancilene Santos de Araujo
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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Charles dos Santos Estevam
  • Charles dos Santos Estevam
  • Laboratory of Biochemistry and Natural Products Chemistry, Department of Physiology, Federal University of Sergipe (UFS), University City Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n Jardim Rosa Elze, São Cristóvão, Sergipe, 49100-000, Brazil
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  •  Received: 20 October 2015
  •  Accepted: 18 February 2016
  •  Published: 22 April 2016

Abstract

Bowdichia virgilioides is used by the population for treating inflammation in general. This study evaluated the antinociceptive activity and possible mechanisms of action of hydroethanolic extract (HEE) and hexane (HXF), chloroform (CLF), ethyl acetate (EAF) and hydromethanol (HMF) fraction of HEE obtained from the plant stem bark against orofacial pain, as well as in vitro its antioxidant effect on the scavenging of free radical DPPH•. The antioxidant activity of the extract and fractions was evaluated against DPPH• at concentrations of 5, 15, and 25 μg.mL-1 for each sample studied, with gallic acid used as positive control. The absorbance decrease was spectrophotometrically measured at 515 nm up to 60 min to obtain the percentage of inhibition of the free radical. The antinociceptive activity was investigated in Swiss mice treated orally with HEE, EAF and HMF (100, 200, and 400 mg/kg) and morphine (5 mg/kg), using formalin, glutamate and capsaicin orofacial pain models. HEE, EAF and HMF showed the best results regarding the reduction of the DPPH radical (25 μg/mL, 60 min), with percent inhibition of 42.89, 78.52 and 54.96%, respectively. HEE, EAF and HMF significantly (p < 0.001) reduced orofacial nociception in mice in the first (56, 49 and 19%, respectively) and second phases (61, 71 and 69%, respectively) of the formalin pain model, as well as glutamate (82, 69 and 39%, respectively) and capsaicin (49, 68 and 64%, respectively) assays. Animals showed no significant changes in motor performance after treatment with HEE, EAF and HMF in the Rota rod test. In general, the potential of B. virgilioides to treat orofacial pain in its central and peripheral components was confirmed, with HEE, EAF and HMF (mainly at 200 and 400 mg/kg) showing antinociceptive effect in three different orofacial pain models related to opioid, glutamatergic and vanilloid receptors. In addition, it is also possible that their antioxidant activity may be related to the observed antinociceptive effect by reducing the biosynthesis of ROS and other inflammatory mediators.

 

Key words: Bowdichia virgilioides; black sucupira; antioxidant activity; orofacial pain; antinociception; free radical.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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