Antibacterial and antifungal activities of indican (indoxyl -D-glucoside)

Ivanise Brito da Silva; Izabela Lima Rangel; Roberta Maria de Leite Lima; Edeltrudes Oliveira Lima; Paloma Lys de Medeiros; SÃ&nia Pereira Leite

doi:10.5897/AJPP2015.4386

African Journal of
Pharmacy and Pharmacology

Abbreviation: Afr. J. Pharm. Pharmacol.
Language: English
ISSN: 1996-0816
DOI: 10.5897/AJPP
Start Year: 2007
Published Articles: 2285

Full Length Research Paper

Antibacterial and antifungal activities of indican (indoxyl Î²-D-glucoside)

Ivanise Brito da Silva

Ivanise Brito da Silva
LaboratÃ³rio de Cultura de CÃ©lulas, Departamento de Histologia e Embriologia , Universidade Federal de Pernambuco, Recife, Brasil.
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Izabela Lima Rangel

Izabela Lima Rangel
LaboratÃ³rio de Cultura de CÃ©lulas, Departamento de Histologia e Embriologia , Universidade Federal de Pernambuco, Recife, Brasil.
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Roberta Maria de Leite Lima

Roberta Maria de Leite Lima
LaboratÃ³rio de Cultura de CÃ©lulas, Departamento de Histologia e Embriologia , Universidade Federal de Pernambuco, Recife, Brasil.
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Edeltrudes Oliveira Lima

Edeltrudes Oliveira Lima
LaboratÃ³rio de Micologia, Departamento de CiÃªncias FarmacÃªuticas, Universidade Federal da ParaÃba, JoÃ£o Pessoa, Brasil.
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Paloma Lys de Medeiros

Paloma Lys de Medeiros
LaboratÃ³rio de Cultura de CÃ©lulas, Departamento de Histologia e Embriologia , Universidade Federal de Pernambuco, Recife, Brasil.
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SÃ´nia Pereira Leite

SÃ´nia Pereira Leite
LaboratÃ³rio de Cultura de CÃ©lulas, Departamento de Histologia e Embriologia , Universidade Federal de Pernambuco, Recife, Brasil.
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Article Number - 496BA3257505
Vol.10(11), pp. 200-205 , March 2016
https://doi.org/10.5897/AJPP2015.4386

Received: 20 June 2015
Accepted: 28 January 2016
Published: 22 March 2016

Abstract

Indican (Indoxyl β-d-glucoside), plant pigments found in true Indigo, are present in Indigofera genus plants, fungi and human urine. This study evaluated the antibacterial and antifungal activities of this pigment using the microdilution method. Antibacterial and antifungal activities were performed against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Candida albicans, Candida tropicalis, Candida krusei, Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseun. Minimum inhibitory concentrations (MICs) were measured according to the broth microdilution protocols by the Clinical and Laboratory Standards Institute (CLSI). The Indican showed only antifungal activity. It had MIC values of 128 µg/mL for yeasts and 512 µg/mL for most of the dermatophytes. The minimum fungicidal concentration (MFC) values of the Indican ranged between 512 μg/mL: C. tropicalis (LM-6) and C. krusei (LM-6 and LM-8) and 1,024 μg/mL: C. albicans (ATCC-76645, LM-108, LM-P20). Further studies are needed to clarify the potential fungicidal activity of the Indican and the possibility for topical applications.