African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Photoprotective, antibacterial activity and determination of phenolic compounds of Neoglaziovia variegata (Bromeliaceae) by high performance liquid chromatography-diode array detector (HPLC-DAD) analysis

Raimundo Goncalves de Oliveira-Junior
  • Raimundo Goncalves de Oliveira-Junior
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Grasielly Rocha Souza
  • Grasielly Rocha Souza
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Amanda Leite Guimaraes
  • Amanda Leite Guimaraes
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Ana Paula de Oliveira
  • Ana Paula de Oliveira
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Camila de Souza Araujo
  • Camila de Souza Araujo
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Juliane Cabral Silva
  • Juliane Cabral Silva
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Alessandra Gomes Marques Pacheco
  • Alessandra Gomes Marques Pacheco
  • Universidade Estadual de Feira de Santana, 44.031-160, Feira de Santana, Bahia, Brazil.
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Sarah Raquel Gomes de Lima-Saraiva
  • Sarah Raquel Gomes de Lima-Saraiva
  • Universidade Federal de Pernambuco, 50.670-901, Recife, Pernambuco, Brazil.
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Larissa Araujo Rolim
  • Larissa Araujo Rolim
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Pedro Jose Rolim Neto
  • Pedro Jose Rolim Neto
  • Universidade Federal de Pernambuco, 50.670-901, Recife, Pernambuco, Brazil.
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Rosane Nora Castro
  • Rosane Nora Castro
  • Universidade Federal Rural do Rio de Janeiro, 23.890-000, Seropedica, Rio de Janeiro, Brazil.
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Jackson Roberto Guedes da Silva Almeida*
  • Jackson Roberto Guedes da Silva Almeida*
  • Universidade Federal do Vale do Sao Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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  •  Received: 07 March 2015
  •  Published: 15 June 2015

Abstract

The photoprotective activity of flowers and antibacterial activity of the leaves of Neoglaziovia variegata were investigated. This study also identified the first phenolic compounds of the extracts and fractions of this species by high performance liquid chromatography (HPLC). The photoprotective activity was measured using the spectrophotometric method. The antibacterial activity was evaluated by microdilution method. The chromatographic analysis was performed in HPLC coupled with iode array detector (DAD) detector, using wavelengths of 254 and 320 nm. Nv-Fl-EtOH, Nv-Fl-CHCl3 and Nv-Fl-AcOEt showed characteristic absorption bands in regions UVB and UVA in a concentration-dependent manner. Nv-Fl-CHCl3 presented the highest sun protection factor (11.45 ± 2.87). Extracts from the leaves showed activity against most of the microorganisms tested, especially Bacillus cereus, Escherichia coli, Salmonella enterica, Serratia marcescens and Shigella flexneri. HPLC analysis revealed the presence of six phenolics: two flavonoids (isoquercetin and kaempferol-3-O­-rhamnoside) and four phenolic acids (caffeic, protocatechuic, p-coumaric and vanillic acids). This result justifies the biological properties, that the species has demonstrated and can lead other phytochemical studies aiming the isolation of these compounds.

Key words: Bromeliaceae, Neoglaziovia variegata, photoprotective activity, antibacterial activity, phenolic compounds.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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