African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Antibacterial activities of Bidens pilosa L, Hoslundia opposita Vahl, and Ageratum conyzoides L against some common wound pathogens

Catherine Namuga
  • Catherine Namuga
  • Department of Polymer, Industrial and Textile Engineering, Busitema University, P. O. Box 256, Tororo, Uganda.
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Haruna Muwonge
  • Haruna Muwonge
  • Department of Physiology, College of Health Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda.
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Margaret Lubwama
  • Margaret Lubwama
  • Department of Microbiology, College of Health Sciences, Makerere University, P. O. Box 7072, Kampala, Uganda.
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Nakyejwe Janet
  • Nakyejwe Janet
  • Natural Chemotherapeutics Research Institute, Ministry of Health Uganda, P. O. Box 4864, Kampala, Uganda.
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Tahalu Sekulima
  • Tahalu Sekulima
  • Department of Microbiology, Faculty of Veterinary Medicine, Division of Pharmacology, Makerere University, P. O. Box 7062, Kampala, Uganda.
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John Baptist Kirabira
  • John Baptist Kirabira
  • Department of Mechanical Engineering, College of Engineering, Design Art and Technology. Makerere University, P. O. Box 7072, Kampala, Uganda.
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  •  Received: 16 February 2022
  •  Accepted: 30 March 2022
  •  Published: 31 May 2022

Abstract

Bacterial infection cause delayed wound healing, and the rising cases of antibiotic resistance call for alternative solutions. This research aims to determine and compare the antibacterial activity of selected research plants when extracted by different methods using different solvents. The plants were extracted with ethanol, methanol, acetone, water, chloroform, and ethyl acetate using Soxhlet and maceration extraction. Agar well diffusion and resazurin dye reduction method were used to determine the antibacterial activity of the extracts against methicillin-resistant Staphylococcus aureus (MRSA), S. aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Agar well diffusion results showed that water, ethyl acetate (Soxhlet), and 100% ethanol (macerated) extracts for all 3 plants inhibited a broad spectrum of bacteria. MIC results showed that ethanol and 70% ethanol (macerated) extracts of Hoslundia opposita had the lowest MIC (1.95 mg/ml) against S. aureus. The ethanol extract of H. opposita Vahl showed the lowest MIC against MRSA. Water, ethanol, 100% ethyl acetate (macerated) and 70% ethanol (macerated) extracts of Ageratum conyzoides showed the lowest MIC against E. faecalis. Ethyl acetate extract of A. conyzoides showed the lowest MIC against E. coli. 100% methanol (macerated) extract of H. opposita showed the lowest MICs against K. pneumoniae and P. aeruginosa, respectively; All plants showed better antibacterial activity on gram positive than gram negative bacteria. The methanol extract of H. opposita showed better activity than the rest of the extracts.

Key words: Ageratum conyzoides L., antibacterial activity, Bidens pilosa L., Hoslundia opposita Vahl, wound healing.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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