African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2280

Full Length Research Paper

Biological activities of the hydro-alchoholic and aqueous extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan

Hana M. Hammad1*, Camelia Albu2, Suzan A. Matar1, Simona-Carmen Litescu2, Hala I. Al Jaber3, Amal S. Abualraghib4 and Fatma U. Afifi5
1Department of Biology, Faculty of Science, The University of Jordan, Amman 11942, Jordan. 2Centre of Bioanalysis, National Institute for Biolological Sciences, Bucharest, Romania. 3Department of Applied Sciences, Faculty of Engineering Technology, Al-Balqa Applied University, Marka, Amman11134, Jordan. 4Department of Physiology and Biochemistry, Faculty of Medicine, The University of Jordan, Amman 11942, Jordan. 5Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Amman 11942, Jordan.
Email: [email protected]

  •  Accepted: 17 June 2013
  •  Published: 08 July 2013


Aqueous and hydro-alcoholic extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan were screened for their antioxidant, antibacterial and antiplatelet efficacy. Total phenols and flavonoids were determined colorimetrically. The radical scavenging activities were evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity assays. Antimicrobial activities were determined by the disc-diffusion method, and the minimum inhibition concentration and the minimum bactericidal concentration tests. Gram positive bacteria presented sensitivity to the hydro-alcoholic extract in the disk diffusion test. No significant activity was observed against Gram negative bacteria and Candida albicans. Hydro-alcoholicextract had a bactericidal activity against Streptococcus pneumoniaeBacillus cereus, Enterococcus faecalis and Klebsiella pneumoniae rather than inhibitory effect. In vitroantiplatelet activity was determined with human whole blood using an electrical impedance method. At concentrations (50, 100, and 200 μg/ml), the extracts showed a non-dose dependent enhancement of platelet aggregation induced by adenosine diphosphate (ADP) (51.82, 52.73 and 51.82%, respectively) and collagen (0.00, 11.76 and 23.53%, respectively). High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis resulted in the identification of 8 phenolic compounds; quercetin 3-β-D-glucoside was the main component.


Key words: Achillea biebersteinii, Asteraceae, antioxidant activity, antibacterial activity, antiplatelet activity, Jordan.