African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

GC-MS analysis and in vitro bioactivity of fixed oil and fatty acid fraction obtained from seeds of Simira gardneriana, a Rubiaceae from Brazilian Caatinga Biome

Christiane Adrielly Alves Ferraz
  • Christiane Adrielly Alves Ferraz
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Raimundo Gonçalves de Oliveira-Júnior
  • Raimundo Gonçalves de Oliveira-Júnior
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Érica Martins de Lavor
  • Érica Martins de Lavor
  • Laboratório de Oncologia Experimental (LOEx), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Mariana Gama e Silva
  • Mariana Gama e Silva
  • Laboratório de Oncologia Experimental (LOEx), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Ana Paula de Oliveira
  • Ana Paula de Oliveira
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Amanda Leite Guimarães
  • Amanda Leite Guimarães
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Izabel Cristina Casanova Turatti
  • Izabel Cristina Casanova Turatti
  • Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14.040-903, Ribeirão Preto, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Norberto Peporine Lopes
  • Norberto Peporine Lopes
  • Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14.040-903, Ribeirão Preto, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Rosemairy Luciane Mendes
  • Rosemairy Luciane Mendes
  • Laboratório de Oncologia Experimental (LOEx), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
José Alves de Siqueira-Filho
  • José Alves de Siqueira-Filho
  • Centro de Referência para Recuperação de Áreas Degradadas (CRAD), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Jackson Roberto Guedes da Silva Almeida
  • Jackson Roberto Guedes da Silva Almeida
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar
Xirley Pereira Nunes
  • Xirley Pereira Nunes
  • Núcleo de Estudos e pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
  • Search for this author on:
  • Google Scholar


  •  Received: 12 March 2016
  •  Accepted: 02 June 2016
  •  Published: 22 June 2016

Abstract

Rubiaceae family includes many species with biological activity, highlighting the genus Simira. In the Caatinga, this genus is represented by six species, and among them, Simira gardneriana is the only endemic species. Previous studies with this plant have shown that extracts from the aerial parts have antioxidant and antimicrobial activity. Thus, the aim of this study was to investigate the chemical composition, antibacterial and cytotoxic activities of fixed oil and fatty acids fraction obtained from seeds of the plant. For this, the fixed oil (Si-FO) was obtained through an extraction with petroleum ether. Subsequently, the fraction of fatty acids (Si-FA) was obtained and then esterified to facilitate analysis by gas chromatography-mass spectrometry GC-MS. Si-FO and Si-FA were evaluated for their antibacterial (MIC and MBC determination) and cytotoxic (MTS assays) properties. The GC-MS analysis identified squalene (39.95%), β-sitosterol (13.82%) and palmitic aldehyde (7.02%) as the major components of Si-FO. Meanwhile, the major compounds identified for the methylated fatty acids fraction were oleic (51.17%), 5,6-octadecadienoic (16.22%) and stearic acids (10.47%). In terms of biological activity, Si-FO and Si-FA exhibited relevant antibacterial activity against Enterococcus faecalis, Escherichia coli and Bacillus cereus strains. In addition, Si-FO showed moderate cytotoxicity against Sarcome S-180 cells, reaching 50.58% of cytotoxic activity in the highest concentration tested (400 μg/ml). These results can be explained by the chemical composition of the samples, since previous studies reported antibacterial and cytotoxic effects of the major compounds identified in Si-FO and Si-FA.

Key words: Fixed oil, fatty acids, antibacterial, cytotoxic, Rubiaceae, Simira, Caatinga.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 23
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES