Nitrofurantoin is a broad-spectrum bactericidal antibiotic which affect both Gram-negative and Gram-positive bacteria. Nitrofurantoin exhibits bacteriostatic or bactericidal effects by inhibiting the synthesis of DNA, RNA, protein and cell wall synthesis. Nanocrystals of NFT were prepared by Cold High Pressure Homogenization technique. NFT was dispersed in aqueous surfactant solution containing Poloxamer 188, PVPK 30 and HPMC E3 under continuous stirring. Poloxamer 188 was used as a surfactant for the preparation of the NCs Formulation. NC9B3 have mean particle size 231 ± 9 nm with Polydispersity Index 0.09 ± 0.02 which indicates very narrow particle size distribution. % Entrapment efficiency was 98.3± 0.7 and drug release profile indicates the homogeneous dispersion of NFT in lipid matrix. NCs have crystalline nature with rough surfaces which was confirmed by SEM analysis. XRPD spectra show the reduction in crystalline behavior of the drug in NCs. There was no significant change in the mean particle size and polydispersity index after 6 months storage at 25°C/60% RH

Keywords: Nitrofurantoin (NFT), Nanocrystals (NCs)