African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Variation of antioxidant and antibacterial activities of ethanolic extracts of propolis in three bee-keeping agro-ecological zones of Uganda

Julius Kyomya
  • Julius Kyomya
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Mariam Kaanyi Kirabo
  • Mariam Kaanyi Kirabo
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Wilberforce John Mayoka
  • Wilberforce John Mayoka
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Rehema Namunyenga
  • Rehema Namunyenga
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Rogers Jaggwe
  • Rogers Jaggwe
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Lawrence Imanirampa
  • Lawrence Imanirampa
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar
Jonans Tusiimire
  • Jonans Tusiimire
  • Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P. O. Box 1410, Mbarara, Uganda.
  • Google Scholar


  •  Received: 15 September 2020
  •  Accepted: 28 October 2020
  •  Published: 31 July 2023

References

Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T (2007). Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry 101(4):1383-1392. 
Crossref

 

Al Naggar Y, Sun J, Robertson, A., Giesy JP, Wiseman S (2016). Chemical characterization and antioxidant properties of Canadian propolis. Journal of Apicultural Research 55(4):305-314. 
Crossref

 

Alday-Provencio S, Diaz G, Rascon L, Quintero J, Alda E, Robles-Zepeda R, Garibay-Escobar A, Astiazaran H, Hernandez J, Velazquez C (2015). Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites. Planta Medica 81(09):742-747. 
Crossref

 

Auamcharoen W, Phankaew C (2016). Antibacterial activity and phenolic content of propolis from four different areas of Thailand. International Journal of Pharmaceutical Sciences Review and Research 37: 77-82.

 

BakamaNume BB (2011). A Contemporary Geography of Uganda. Oxford: African Books Collective.

View

 

Bankova V, Popova M, Trusheva B (2014). Propolis volatile compounds: chemical diversity and biological activity: a review. Chemistry Central Journal 8(1):28.
Crossref

 

Bendel JL (2002). Characterisation of the normal flora of honeybee hives in Central Wisconsin. Univ Wisconsin-La Crosse Journal of Undergraduate Research 437:441.

 

Brugger D, Krondorfer I, Zahma K, Stoisser T, Bolivar JM, Nidetzky B, Peterbauer CK, Haltrich D (2014). Convenient microtiter plate?based, oxygen?independent activity assays for flavin?dependent oxidoreductases based on different redox dyes. Biotechnology Journal 9(4):474-482. 
Crossref

 

Christov R, Trusheva B, Popova M, Bankova V, Bertrand M (2006). Chemical composition of propolis from Canada, its antiradical activity and plant origin. Natural Product Research 20(06):531-536.
Crossref

 

Daleprane JB, Abdalla DS (2013). Emerging roles of propolis: antioxidant, cardioprotective, and antiangiogenic actions. Evidence-Based Complementary and Alternative Medicine 2013.
Crossref

 

Drago L., Mombelli B, Vecchi ED, Tocalli MFL, Gismondo MR (2000). In vitro antimicrobial activity of propolis dry extract. Journal of Chemotherapy 12(5):390-395. 
Crossref

 

Fernandes Jr A, Sugizaki M, Fogo M, Funari S, Lopes C (1995). In vitro activity of propolis against bacterial and yeast pathogens isolated from human infections. Journal of Venomous Animals and Toxins 1(2):63-69. 
Crossref

 

Garedew A, Schmolz E, Lamprecht I (2004). Microbiological and calorimetric investigations on the antimicrobial actions of different propolis extracts: an in vitro approach. Thermochimica Acta 422(1-2):115-124. 
Crossref

 

Gonsales G, Orsi R, Fernandes Júnior A, Rodrigues P, Funari S (2006). Antibacterial activity of propolis collected in different regions of Brazil. Journal of Venomous Animals and Toxins Including Tropical Diseases 12(2):276-284. 
Crossref

 

Hamasaka T, Kumazawa S, Fujimoto T, Nakayama T (2004). Antioxidant activity and constituents of propolis collected in various areas of Japan. Food Science and Technology Research 10(1):86-92. 
Crossref

 

Huang S, Zhang CP, Wang K, Li GQ, Hu FL (2014). Recent advances in the chemical composition of propolis. Molecules 19(12):19610-19632. 
Crossref

 

Kajobe R, Agea JG, Kugonza DR, Alioni V, Otim SA, Rureba T, Marris G (2009). National beekeeping calendar, honeybee pest and disease control methods for improved production of honey and other hive products in Uganda. National Agricultural Research Organisation, Entebee.

 

Khacha-Ananda S, Tragoolpua K, Chantawannakul P, Tragoolpua Y (2013). Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods. Asian Pacific Journal of Cancer Prevention 14(11):6991-6995. 
Crossref

 

Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S (1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology 64(3):235-240. 
Crossref

 

Kumazawa S, Hamasaka T, Nakayama T (2004). Antioxidant activity of propolis of various geographic origins. Food Chemistry 84(3):329-339. 
Crossref

 

Kuropatnicki AK, Szliszka E, Krol W (2013). Historical aspects of propolis research in modern times. Evidence-Based Complementary and Alternative Medicine 2013. 
Crossref

 

Lagouri V, Prasianaki D, Krysta F (2014). Antioxidant properties and phenolic composition of Greek propolis extracts. International Journal of Food Properties 17(3):511-522. 
Crossref

 

Lu LC, Chen YW, Chou CC (2003). Antibacterial and DPPH free radical-scavenging activities of the ethanol extract of propolis collected in Taiwan. Journal of Food and Drug Analysis 11(4):277-282.
Crossref

 

Marcucci M, Ferreres F, Garc?a-Viguera C, Bankova V, De Castro S, Dantas A, Valente P, Paulino N (2001). Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology 74(2):105-112. 
Crossref

 

Massaro FC, Brooks PR, Wallace HM, Nsengiyumva V, Narokai L, Russell FD (2013). Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries. PLoS One 8(11):e81297. 
Crossref

 

Katircioglu H, Mercan N (2006). Antimicrobial activity and chemical compositions of Turkish propolis from different regions. African Journal of Biotechnology 5(11):1151-1153.

 

Kronvall G (1982). Analysis of a single reference strain for determination of gentamicin regression line constants and inhibition zone diameter breakpoints in quality control of disk diffusion antibiotic susceptibility testing. Journal of Clinical Microbiology 16(5):784-793.
Crossref

 

Kumazawa S, Hamasaka T, Nakayama T (2004). Antioxidant activity of propolis of various geographic origins. Food Chemistry 84(3):329-339. 
Crossref

 

Muli E, Maingi J (2007). Antibacterial activity of Apis mellifera L. propolis collected in three regions of Kenya. Journal of Venomous Animals and Toxins including Tropical Diseases 13(3):655-663. 
Crossref

 

Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hara H (2009). Comparison of bee products based on assays of antioxidant capacities. BMC Complementary and Alternative Medicine 9(1):4. 
Crossref

 

Nina N, Lima B, Feresin GE, Gimenez A, Salamanca Capusiri E, Schmeda-Hirschmann G (2016). Antibacterial and leishmanicidal activity of Bolivian propolis. Letters in Applied Microbiology 62(3):290-296. 
Crossref

 

Nina N, Quispe C, Jimenez-Aspee F, Theoduloz C, Feresin GE, Lima B, Leiva E, Schmeda-Hirschmann G (2015). Antibacterial activity, antioxidant effect and chemical composition of propolis from the Region del Maule, Central Chile. Molecules 20(10):18144-18167. 
Crossref

 

Ory EM, Yow EM (1963). The use and abuse of the broad spectrum antibiotics. JAMA 185(4):273-279. 
Crossref

 

Runyoro DK, Ngassapa OD, Kamugisha A (2017). Antimicrobial activity of propolis from Tabora and Iringa regions, Tanzania and synergism with gentamicin. Journal of Applied Pharmaceutical Science 7(01):171-176. 
Crossref

 

Seidel V, Peyfoon E, Watson DG, Fearnley J (2008). Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytotherapy Research 22:1256-1263. 
Crossref

 

Sforcin J, Fernandes Jr A, Lopes C, Bankova V, Funari S (2000). Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology 73(1-2):243-249. 
Crossref

 

Siheri W, Alenezi S, Tusiimire J, Watson DG (2017). The chemical and biological properties of propolis. In Bee Products-Chemical and Biological Properties. Springer, Cham. pp. 137-178.
Crossref

 

Siheri W, Igoli JO, Gray AI, Nasciemento TG, Zhang T, Fearnley J, Clements CJ, Carter KC, Carruthers J, Edrada?Ebel R, Watson DG (2014). The isolation of antiprotozoal compounds from Libyan propolis. Phytotherapy Research 28(12):1756-1760. 
Crossref

 

Siheri W, Zhang T, Ebiloma GU, Biddau M, Woods N, Hussain MY, Clements CJ, Fearnley J, Ebel RE, Paget T, Muller S (2016). Chemical and antimicrobial profiling of propolis from different regions within Libya. PLoS ONE 11(5):e0155355. 
Crossref

 

Socha R, Galkowska D, Bugaj M, Juszczak L (2015). Phenolic composition and antioxidant activity of propolis from various regions of Poland. Natural Product Research 29(5):416-422. 
Crossref

 

Sulaiman GM, Al Sammarrae KW, Ad'hiah AH, Zucchetti M, Frapolli R, Bello E, Erba E, D'incalci M, Bagnati R (2011). Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials. Food and Chemical Toxicology 49(9):2415-2421. 
Crossref

 

Taddeo VA, Epifano F, Fiorito S, Genovese S (2016). Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis. Journal of Pharmaceutical and Biomedical Analysis 129:219-223. 
Crossref

 

Toreti VC, Sato HH, Pastore GM, Park YK (2013). Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-Based Complementary and Alternative Medicine 2013. 
Crossref

 

The Uganda National Apiculture Development Organisation (TUNADO) (2015). A Market Information Report on Hive Products in Uganda. The Uganda National Apiculture Development Organisation, 13 p.

 

Uganda Export Promotion Board (UEPB) (2005). A Report by Uganda Export Promotion Board (UEPB) and the Sector Counterpart Team. Uganda Apiculture Export Strategy P 34.

 

US Marine Corps Uganda (2014). Marine Corps Intelligence Activity Uganda Country Handbook | Public Intelligence.

View

 

Vargas-Sanchez RD, Torrescano-Urrutia GR, Acedo-Felix E, Carvajal-Millan E, Gonzalez-Cordova AF, Vallejo-Galland B, Torres-Llanez MJ, Sanchez-Escalante A (2014). Antioxidant and antimicrobial activity of commercial propolis extract in beef patties. Journal of Food Science 79(8):C1499-C1504. 
Crossref

 

Yildirim A, Duran GG, Duran N, Jenedi K, Bolgul BS, Miraloglu M, Muz M (2016). Antiviral activity of Hatay propolis against replication of Herpes simplex virus type 1 and type 2. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 22:422-430.
Crossref

 

Zhang T, Omar R, Siheri W, Al Mutairi S, Clements C, Fearnley J, Edrada-Ebel R, Watson D (2014). Chromatographic analysis with different detectors in the chemical characterisation and dereplication of African propolis. Talanta 120:181-190. 
Crossref

 

Zhang JL, Wang K, Hu FL (2013). Advance in studies on antioxidant activity of propolis and its molecular mechanism. Zhongguo Zhong yao za zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica 38(16):2645-2652.