Full Length Research Paper
Abstract
Variation in transmission of ten clones of Schizaphis graminum (Rondani) originating from different regions of Iran was evaluated by transmitting an isolate of barley yellow dwarf virus-PAV (BYDV-PAV) for which this species is normally an inefficient vector. After acquiring the virus, aphids of each clone-virus isolate combination were caged on 30 seedling using three forth instar nymphs per seedling. The inoculated and control plants were assayed by ELISA and assayed visually to determine the rate of transmission and the mean ELISA value for each treatment. A positive correlation was found between the rate of transmission and the mean absorbance value. Aphid clones differed in their efficiency of transmission of virus isolate. On the other hand, single clone transmitted virus isolate with varying efficiencies. The aphid clone of virus isolate origin transmitted virus isolate at the highest rate. In general, Shahre kord and Shahreza clones of aphids showed the highest efficiency of transmission and Delijan and Varamin clones had the lowest transmission efficiency between aphid clones.
Key words: Luteovirus, aphid clones, BYDV-PAV, transmission efficiency, Shizaphis graminum, Iran.
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