African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 791

Full Length Research Paper

In vitro propagation of Ceropegia juncea Roxb

Krishnareddy P. V.1, Karuppusamy S.2 and Pullaiah T.1*        
1Department of Botany, Sri Krishnadevaraya University, Anantapur‐515 003, Andhra Pradesh, India.  2Department of Botany, The Madura College, Madurai, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 26 November 2011


The present study was conducted to establish a protocol for in vitro propagation of an endemic medicinal plant Ceropegia juncea (Asclepiadaceae) from one month old aseptic seedling nodal explants. The highest shoot multiplication rate of 20.65 ± 0.20 shoots/explant was achieved on Murashige and Skoog medium supplemented with BAP 8.87 mM + TDZ  4.54 mM. Excised shoots were rooted on half strength MS medium with IBA 4.90 mM + NAA 1.27 mM. 78% of the rooted shoots survived in the field.


Key words: Ceropegia juncea, in vitro propagation, endemic medicinal plant, nodal explants, multiple shoots.