African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 791

Full Length Research Paper

Change of some defense compounds of cucumber treated with Bacillus cereus and salicylic acid against Meloidogyne javanica

Sara Siahpoush1,2*, Navazollah Sahebani2 and Heshmatollah Aminian2      
1Plant Pathology Abouryhan Campus, University of Tehran, Iran. 2653, Azadi lane, Postal code: 68168 13898, Enghelab Boulevard, Khorramabad, Lorestan, Iran. 3Department of Plant Protection, Abouryhan Campus, University of Tehran, Iran.  
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 27 November 2011


In this research, the effects of antagonist bacterium (Bacillus cereus) and salicylic acid (SA) as chemical inducer alone and in combination against root knot nematode (Meloidogyne javanica) as the most important nematode in Iran, on cucumber roots (cv. superdominus) was investigated. The used concentrations were 109 cfu/ml for bacteria and 5 mM for SA. In greenhouse experiments some parameters were evaluated including nematode and plant growth criteria in treated plants with the two above materials. Results of this experiment showed that combination of biocontrol agent and chemical inducer decreased galling, egg masses and egg numbers on roots of cucumber. In biochemical analysis, also, some of defense compounds such as hydrogen peroxide and peroxidase activities were measured. Results showed that hydrogen peroxide level and peroxidase activity increased after inoculation until 4th and 5th days respectively and then decreased gradually. Soil drenching of B. cereus and SA caused maximum induction in roots.


Key words: Bacillus cereus, salicylic acid, Meloidogyne javanica, peroxidase, hydrogen peroxide.