African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 804

Full Length Research Paper

Offspring of the crosses of two anti-sense potato plants exhibit additive cellulose reduction

Olawole O. Obembe1,2
  1Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ Wageningen, The Netherlands. 2Department of Biological Sciences, College of Science and Technology, Covenant University, P. M. B. 1023 Ota, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 30 October 2008
  •  Published: 31 December 2010

Abstract

 

Two transgenic potato lines, csr2-1 and csr4-8, containing two different antisense genes: csr2 and csr4, respectively, were crossed to investigate the possibility of achieving reduction in cellulose content in the tuber cell walls of the progeny. The progeny containing both transgenes (double csr2/csr4 transformant) exhibited reduction of 63% in cell wall cellulose content, while the single transformants csr2 and csr4 had a 44 and 22% reduction in cellulose content, respectively.

 

Key words: Cellulose content, cellulose synthase, double transformant, genetic crossing, Solanum tuberosum.