International Journal of
Biodiversity and Conservation

  • Abbreviation: Int. J. Biodivers. Conserv.
  • Language: English
  • ISSN: 2141-243X
  • DOI: 10.5897/IJBC
  • Start Year: 2009
  • Published Articles: 679

Full Length Research Paper

In vitro conservation of exotic potato genotypes through different Incubated temperatures, aerophilic and micro-aerophilic conditions

Shahid Ali
  • Shahid Ali
  • Department of Botany, Hazara University, Mansehra, Pakistan.
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Shazia Erum
  • Shazia Erum
  • National Agricultural Research Center Islambad, Pakistan
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Faisal Nouroz
  • Faisal Nouroz
  • Department of Botany, Hazara University, Mansehra, Pakistan. 2National Agricultural Research Center Islambad, Pakistan
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Naeem Khan
  • Naeem Khan
  • Department of Plant Sciences Quaid-i-Azam University, Islamabad, Pakistan.
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Asif Mehmood
  • Asif Mehmood
  • Department of Botany, Abdul Wali Khan University, Mardan, Pakistan.
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Sayed Haider Ali Shah
  • Sayed Haider Ali Shah
  • Department of Botany, Hazara University, Mansehra, Pakistan. 2National Agricultural Research Center Islambad, Pakistan
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Aamir Raheem
  • Aamir Raheem
  • Biological Science, Shaukat Khanum Memorial Hospital and Research Center, Pakistan.
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Aish Muhammad
  • Aish Muhammad
  • National Agricultural Research Center Islambad, Pakistan
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  •  Received: 14 March 2016
  •  Accepted: 28 May 2016
  •  Published: 31 July 2016

Abstract

The present study was carried out to study the in vitro conservation of potato genotypes at different temperatures and aerophilic and micro aerophilic conditions. A total of 31 genotypes were conserved at different incubated temperature ranging from 10 to 25°C. At lower temperature (10°C) plant growth was slowest as compared to plants incubated at high temperature (25°C). The results revealed that aerophilic condition was optimum for the growth of all potato genotypes. Data were collected on plant height, number of roots and number of nodes. Maximum plant height, highest number of roots and number of nodes were observed in all genotypes grown at 25°C. In vitro microareophillic condition of the plant growth was very slow but conservation was maximum. It was concluded from the present investigation that low temperature and micro-aerophilic condition is best for in vitro conservation of International Potato Center (CIP) germplasm which can increase the period between sub culturing.

Key words: In vitro conservation, genotype, sub-culturing, aerophilic.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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