Full Length Research Paper
Abstract
The aim of the present study was to evaluate the antiacetylcholinesterase and antioxidant potential of 80% methanolic extract of Rhododendron yedoense var. poukhanense bark (RYBE). The extract was rich in total phenolic content (160 mg gallic acid equivalent/g of dry extract) and possessed flavonoids (2.2 mg quercetin equivalent/g of dry extract). The acetylcholinesterase (AChE) inhibitory capacity of RYBE was determined against electric eel AChE enzyme. The extract exhibited substantial inhibitory activity with IC50 value of 169.01 µg/ml. In addition, the extract revealed its strong antioxidant activity by various in vitro assay systems including 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), ferrous ion chelating and nitric oxide scavenging assays. The reducing power of RYBE was also determined by ferric reducing antioxidant power assay. Furthermore, this extract exhibited dose dependent inhibition of lipid peroxidation in rat liver homogenate. In conclusion, due to considerable AChE inhibitory and potent antioxidant nature of RYBE, further in vivo studies along with structural elucidation to find the active ingredient(s) will prove useful in searching an effective treatment for Alzheimer’s disease.
Key words: Acetylcholinesterase, antioxidant, Ferric (Fe3+) reducing antioxidant power (FRAP), free radical, Rhododendron yedoense var. Poukhanense bark, total flavonoid content, total phenolic content.
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