Abstract

Two glycosylated flavonoids cyanidin-3- glycosides and quercetin-3-rutinoside were isolated from mango leaves, were studied for Aulacophora foeveicollis cellulase inhibition studies. The glycosylated flavonoids cyanidin-3- glucosides (C3G) was able to completely inhibit the enzyme while quercetin,3-(6-o-alpha-l-rhamnopyranosyl-beta-d-glucopyranoside) (Rutin) inhibited to a lesser extant. Rutin and C3G proven to be the non-competitive inhibiters and had same inhibitor constant Ki 0.5uM, indicating the structural similarity in the binding site. Rutin and C3G were also able to retard the growth of two bacterial strains Pseudomonas and Staphylococcus (isolated as associated microbes of Aulacophora). It is concluded that the natural glycosylated flavonoids stands good merit as, potential insect cellulose hydrolyzing enzyme inhibitors.

Key words: Endo-beta-1, 4-glucanase inhibition, Mangifera indica, Aulacophora fovecollis, Quercetin, Cyanidin -3-glucoside (C3G), Quercetin-3-rutinoside.