Abstract

Oxidative stress has been reported to be associated with cellular injury. Here, we investigated the antioxidant ability of a novel peptide mixture purified from black soybean (Rhynchosia volubilis) that exhibited significant antioxidant ability (IC₅₀ = 119.8 ± 1.2 µg/ml) under in vitro ABTS assay conditions. To confirm this result, we next examined the antioxidant ability of the peptide mixture using cell culture systems. Black soybean peptide (BSP) significantly reduced the H₂O₂-induced cell death in C2C12 myocyte. Moreover, extracellular signal-regulated kinase (ERK) signaling was activated by BSP treatment. Under the same conditions, inhibition of the ERK signaling pathway with PD98059 significantly reduced the antioxidant ability of BSP in C2C12 cells. Finally, we also assessed the antithrombotic ability of BSP using a rat model; we found that the BSP also exerted antithrombotic effects in rat blood. Collectively, these results indicate that BSP purified from black soybean exerts an inhibitory effect on both oxidative stress and thrombosis.

Key words: Black soybean peptide (BSP), anti-oxidant, thrombosis, extra cellular signal regulated protein kinase, oxidative stress.