Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 234

Full Length Research Paper

Topical wound healing activity of an ointment based hydroethanolic leaf extract of Anogeissus leiocarpus

Komlan Tchalla
  • Komlan Tchalla
  • Laboratory of Biomedical, Food and Environmental Health Sciences (LaSBASE), Higher School of Biological and Food Techniques (ESTBA), University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Yendubé T. Kantati
  • Yendubé T. Kantati
  • Pathophysiology, Bioactive Substances and Safety Research Unit (PSBI) - Physiology-Pharmacology Laboratory, Faculty of Sciences, University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Yao Hoekou
  • Yao Hoekou
  • Laboratory of Biomedical, Food and Environmental Health Sciences (LaSBASE), Higher School of Biological and Food Techniques (ESTBA), University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Pascaline Kindji Kpoyizoun
  • Pascaline Kindji Kpoyizoun
  • Pathophysiology, Bioactive Substances and Safety Research Unit (PSBI) - Physiology-Pharmacology Laboratory, Faculty of Sciences, University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Pierre Kokou Afankoutche
  • Pierre Kokou Afankoutche
  • Pathophysiology, Bioactive Substances and Safety Research Unit (PSBI) - Physiology-Pharmacology Laboratory, Faculty of Sciences, University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Mamatchi Melila
  • Mamatchi Melila
  • Laboratory of Process Engineering and Natural Resources, Faculty of Sciences, University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Povi Lawson-Evi
  • Povi Lawson-Evi
  • Pathophysiology, Bioactive Substances and Safety Research Unit (PSBI) - Physiology-Pharmacology Laboratory, Faculty of Sciences, University of Lomé, 01 BP 1515 Lomé 01, Togo.
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Tchadjobo Tchacondo
  • Tchadjobo Tchacondo
  • Laboratory of Biomedical, Food and Environmental Health Sciences (LaSBASE), Higher School of Biological and Food Techniques (ESTBA), University of Lomé, 01 BP 1515 Lomé 01, Togo.
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  •  Received: 16 December 2023
  •  Accepted: 16 January 2024
  •  Published: 31 March 2024

Abstract

Anogeissus leiocarpus is a plant used in the local West African pharmacopeia. This study aimed to evaluate the healing properties of the hydro-ethanol extract of its leaves in Wistar rats. A 1.5 cm diameter excision wound model was used. Wistar rats were treated post-excision by the topical application of petroleum jelly alone, also known as 'Vaseline', petroleum jelly supplemented with 3% (ALHE-3%) and 10% (ALHE-10%) of A. leiocarpus leaves' hydroethanolic extract, or sulfadiazine (reference drug), or no treatment (untreated or Control group). Photographs were taken after excision. Wound surfaces were assessed daily using ImageJ software. Biopsies were taken for histological examination of the excised skin and hydroxyproline determination. The results showed a significant reduction in wound surfaces of animals treated with ALHE-3% and ALHE-10% (33.10 ± 2.54 and 31.14 ± 1.71 mm2, respectively) as early as the sixth day after excision. The dosage of hydroxyproline showed a significant difference in collagen production in animals treated with ALHE-3% and ALHE-10%, compared to the Control (P < 0.001) and Vaseline (P < 0.01) groups at day 14. Histological analysis showed rapid re-epithelialization in animals treated with the extracts. A. leiocarpus leaves' hydroethanolic extract could be a potential natural remedy for treating wounds.

Key words: Anogeissus leiocarpus, excision wound, healing, Wistar rat, hydroxyproline.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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