Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 234

Full Length Research Paper

UHPLC-DAD characterization of bioactive secondary metabolites from Ocimum americanum and Pupalia lappacea extracts: Antioxidant activity and antihypertensive effects on L-NAME-induced hypertensive rats

Adjileye Rafatou Adédoyin Adjokè
  • Adjileye Rafatou Adédoyin Adjokè
  • Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin.
  • Google Scholar
Amoussa Abdou Madjid Olatoundé
  • Amoussa Abdou Madjid Olatoundé
  • Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin.
  • Google Scholar
Rafiou Adamou
  • Rafiou Adamou
  • Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin.
  • Google Scholar
Awede Bonaventure
  • Awede Bonaventure
  • Unit of Physiology, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin.
  • Google Scholar
Laleye Anatole
  • Laleye Anatole
  • Unit of Physiology, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin.
  • Google Scholar
Lagnika Latifou
  • Lagnika Latifou
  • Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin.
  • Google Scholar


  •  Received: 21 March 2019
  •  Accepted: 25 June 2019
  •  Published: 31 July 2019

References

Aditya G, Ajay KS (2017). The Pharmacological Potential of Rutin. Saudi Pharmaceutical Journal 25(2):149-164. 
Crossref

 

Adjatin A, Dansi A, Badoussi E, Loko YL, Dansi M, Gbaguidi F, Azokpota P, Ahissou H, Akoègninou A, Akpagana K, Sanni A (2013). Phytochemical screening and toxicity studies of Crassocephalum rubens (Juss. ex Jacq.) S. Moore and Crassocephalum crepidioides (Benth.) S. Moore consumed as vegetable in Benin. Journal of Chemical and Pharmaceutical Research 5(6):160-167. 

 

Agunloye OM, Oboh G, Ademiluyi AO, Ademosun AO, Akindahunsi AA, Oyagbemi AA, Omobowale TO, Ajibade TO, Adedapo AA (2019). Cardio-protective and antioxidant properties of caffeic acid and chlorogenic acid: Mechanistic role of angiotensin converting enzyme, cholinesterase and arginase activities in cyclosporine induced hypertensive rats. Biomedicine and Pharmacotherapy 109:450-458. 
Crossref

 

Amoussa AMO, Sanni A, Lagnika L (2015). Antioxidant activity and total phenolic, flavonoid and flavonol contents of the bark extracts of Acacia ataxacantha. Journal of Pharmacognosy and Phytochemistry 4(2):172-178. 

View

 

Apenteng JA, Agyare C, Adu F, Ayande PG, Boakye YD (2014). Evaluation of wound healing potential of different leaf extracts of Pupalia lappacea. African Journal of Pharmacy and Pharmacology 8(41):1039-1048. 

 

Babál P, Pechánová O, Bernátová I, Stvrtina S (1997). Chronic inhibition of NO synthesis produces myocardial fibrosis and arterial media hyperplasia. Histology and Histopathology 12:623-9. 

View

 

Bekoe EO, Kretchy IA, Sarkodie JA, Okraku A, Sasu C, Degraft A, Twumasi M (2017). Ethnomedicinal Survey of Plants Used for the Management of Hypertension Sold in the Makola Market, Accra, Ghana. European Journal of Medicinal Plants 19(3):1-9. 
Crossref

 

Berkban T, Boonprom P, Bunbupha S, Welbat J, Kukongviriyapan U, Kukongviriyapan V, Pakdeechote P, Prachaney P (2015). Ellagic acid prevents L-NAME-induced hypertension via restoration of eNOS and p47phox expression in rats. Nutrients, 7(7):5265-5280. 
Crossref

 

Beswick RA, Dorrance AM, Leite R, Webb RC (2001). NADH/NADPH oxidase and enhanced superoxide production in the mineralocorticoid hypertensive rat. Hypertension 38(5):1107-1111. 
Crossref

 

Bilanda DC, Dzeufiet PDD, Kouakep L, Aboubakar BFO, Tedong L, Kamtchouing P, Théophile Dimo (2017). Bidens pilosa Ethylene acetate extract can protect against L-NAME-induced hypertension on rats. BMC Complementary and Alternative Medicine 17:479. 
Crossref

 

Birari DAR, Dhulgande GS (2010). Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology 2(8):11-13.

 

Chiou SY, Sung JM, Huang PW, Lin SD (2017). Antioxidant, Antidiabetic, and Antihypertensive Properties of Echinacea purpurea Flower Extract and Caffeic Acid Derivatives Using In Vitro Models. Journal of Medicinal Food 20(2):171-179. 
Crossref

 

Curin Y, Andriantsitohaina R (2005). Polyphenols as potential therapeutical agents against cardiovascular diseases. Pharmacological Reports 57:97-107.

 

Dibala CI, Konaté K, Diao M, Ouédraogo M, Dicko MH (2016). Chemical composition, antioxidant and antibacterial properties of extracts from ocimum americanum L. against multi-resistant food bacteria. World Journal of Pharmacy and Pharmaceutical Sciences 5(12):1549-1567.

 

Dinata DI, Supriadi D, Djafar G, Syerliana S, Wahyu W, Shelvy ES (2015). Effect of Adding Granul Basil (Ocimum americanum) as Antioxidants in Fried Foods. Indonesian Journal of Pharmaceutical Science and Technology 2(1):22-32. 
Crossref

 

Drăgan M, Stan CD, Iacob AT, Dragostin O, Lenuţa P (2018). Ferulic acid: potential therapeutic applications. The Medical-Surgical Journal 122(2):388-395.

 

Elya B, Handayani R, Sauriasari R, Azizahwati A, Uqie SH, Idam TP, Yunita IP (2015). Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV. Pakistan Journal of Biological Sciences 18(6):279-284. 
Crossref

 

Enemali MO, Udedi SC (2018). Comparative evaluation of the protective effect of leaf extracts of Vernonia amygdalina (bitter leaf) and Ocimum canum (curry) on acetaminophen induced acute liver toxicity. Journal of Pharmacognosy and Phytotherapy 10(7):116-125. 
Crossref

 

Gardiner SM, Compton AM, Bennett T, Palmer RM, Moncada S (1990). Control of regional blood flow by endothelium-derived nitric oxide. Hypertension 15:486-492.
Crossref

 

Goli AH, Barzegar M, Sahari MA (2005). Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chemistry 92:521-525. 
Crossref

 

Graciano ML, de Cassia Cavaglieri R, Dellê H, Dominguez WV, Casarini DE, Malheiros DMAC, Noronha IL (2004). Intrarenal renin-angiotensin system is upregulated in experimental model of progressive renal disease induced by chronic inhibition of nitric oxide synthesis. Journal of the American Society of Nephrology 15(7):1805-1815. 
Crossref

 

Hoekou YP, Batawila K, Gbogbo KA, Karou DS, Ameyapoh Y, Souza C (2012). Evaluation des propriétés antimicrobiennes de quatre plantes de la flore togolaise utilisées en médecine traditionnelle dans le traitement des diarrhées infantiles. International Journal of Biological and Chemical Sciences 6(6):3089-3097. 
Crossref

 

Jazy MA, Haïdara M et Sanogo R (2018). Chromatographie sur couche mince et activité antiradicalaire d'extraits de Pupalia Lappacea (L.) Juss. Amaranthaceae. European Scientific Journal 3(14):140-155. 
Crossref

 

Jordão JBR, Porto HKP, Lopes FM, Batista AC, Rocha ML (2017). Protective Effects of Ellagic Acid on Cardiovascular Injuries Caused by Hypertension in Rats. Planta Medica 83(10):830-836. 
Crossref

 

Kang N, Lee JH, Lee W, Ko JY, Kim EA, Kim JS, Heu MS, Kim GH, Jeon YJ (2015). Gallic acid isolated from Spirogyra sp. improves cardiovascular disease through a vasorelaxant and antihypertensive effect. Environmental Toxicology and Pharmacology 39(2):764-772. 
Crossref

 

Karou SD, Tchacondo T, Agassounon M, Tchibozo D, Anani K, Koudouvo K (2011). Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo. Pharmaceutical Biology 1286-1297. 
Crossref

 

Kimura DC, Nagaoka MR, Borges DR et Kouyoumdjian M (2017). Angiotensin II or epinephrine hemodynamic and metabolic responses in the liver of L-NAME induced hypertension and spontaneous hypertensive rats. World Journal of Hepatology 
Crossref

 

Kumar Naresh, Vikas Pruthi (2014). Potential applications of ferulic acid from natural sources. Biotechnology Reports 4:86-93. 
Crossref

 

Kumar RS, Rajkapoor B, Perumal P (2012). Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models. Asian Pacific Journal of Tropical Biomedicine 2(4):256-261. 
Crossref

 

Lagnika LA, Adjileye RA, Yedomonhan H, Amadou BSA, Sanni A (2016). Ethnobotanical survey on antihypertensive medicinal plants in municipality of Ouémé, Southern Benin. Advanced Herbal Medicine 2(3):20-32.

 

Lajous M, Rossignol E, Fagherazzi G, Perquier F, Scalbert A, Clavel-Chapelon F, Boutron-Ruault MC (2016). Flavonoid intake and incident hypertension in women. American Journal of Clinical Nutrition 103(4):1091-1098. 
Crossref

 

Lodi F, Cogolludo A, Duarte J, Moreno L, Coviello A, De Bruno MP, Vera R, Galisteo M, Jiménez R, Tamargo J, Perez-Vizcaino F (2006). Increased NADPH oxidase activity mediates spontaneous aortic tone in genetically hypertensive rats. European Journal of Pharmacology 544(1-3):97-103. 
Crossref

 

Mehnoor F, Chakrapani R (2015). Phytochemical Evaluation and Pharmacological Screening of Ethanolic Leaf Extracts of Erythroxylum Monogynum and Pupalia Lappacea for Hepatoprotective, Nephroprotective, Antihyperlipidemic and Antihyperglycemic Activity in Alloxan- Induced Diabetic Albino Wistar Rats. IOSR Journal of Pharmacy and Biological Sciences 10(6):83-107. 

 

Mohan SC, Balamurugan V, Salini ST, Rekha R (2012). Metal ion chelating activity and hydrogen peroxide scavenging activity of medicinal plant Kalanchoe pinnata. Journal of Chemical and Pharmaceutical Research 4(1):197-202.

 

Naidu PP, Madakka M, Rajesh B (2014). Pupalia lappacea Juss [L]: a review of phytochemistry and therapeutic application. Asian Journal of Pharmaceutical and Clinical Research 7(1):15-18.

 

Ntonga PA, Baldovini N, Mouray E, Mambu L, Belong P, Grellier P (2014). Activity of Ocimum basilicum, Ocimum canum, and Cymbopogon citratus essential oils against Plasmodium falciparum and mature-stage larvae of Anopheles funestus s.s. Parasite 21(33):1-8. 
Crossref

 

Nyadjeu P, Nguelefack-Mbuyo EP, Atsamo AD, Nguelefack TB, Dongmo AB, Kamanyi A (2013). Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in LNAME-induced hypertensive rats. BMC Complementary and Alternative Medicine 13(27):1-10. 
Crossref

 

Oh KS, Ryu SY, Oh BK, Seo HW Kim YS, Lee BH (2008). Antihypertensive, Vasorelaxant, and Antioxidant Effect of Root Bark of Ulmus macrocarpa. Biological and Pharmaceutical Bulletin 31(11): 2090-2096. 
Crossref

 

Orch H, Douira A, Zidane L (2015). Étude ethnobotanique des plantes médicinales utilisées dans le traitement du diabète, et des maladies cardiaques dans la région d'Izarène (Nord du Maroc). Journal of Applied Biosciences 86:7940-7956. 
Crossref

 

Organisation mondiale de la Santé, Journée mondiale de la santé : Panorama mondial de l'Hypertension, Un « tueur silencieux » responsable d'une crise de santé publique mondiale. 2013. WHO/DCO/WHD/2013.2

 

Pandey A, Tripathi S (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry 2(5):115-119.

 

Porteri E, Rizzoni D, De Ciuceis C, Boari GE, Platto C, Pilu A, Miclini M, Agabiti Rosei C, Bulgari G, Agabiti Rosei E (2010). Vasodilator effects of red wines in subcutaneous small resistance artery of patients with essential hypertension. American Journal of Hypertension 23:373-378. 
Crossref

 

Prasad PN, Madakka M, Bandi R (2014). Pupalia Lappacea Juss [L]: A review of phytochemistry and therapeutic application. Asian Journal of Pharmaceutical and Clinical Research 7(1):15-18.

 

Raji NO, Adebisi IM, Bello SO (2013). Survey Of Antihypertensive Agents In Sokoto, Northwest Nigeria. International Journal of Innovative Research and Development 2(5):1820-1835.

 

Rossoni G, Manfredi B, De Gennaro CV, Berti M, Guazzi M, Berti F (2007). Sildenafil reduces L-NAME-induced severe hypertension and worsening of myocardial ischaemia-reperfusion damage in the rat. Brazilian Journal of Pharmacology 150:567-76. 
Crossref

 

Saeed N, Khan MR, Shabbir M (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine 12:221 
Crossref

 

Sarma DSK, Venkata ASB (2011). Pharmacognostic and phytochemical studies of Ocimum americanum. Journal of Chemical and Pharmaceutical Research 3(3):337-347

 

Shah MD, Hossain MA (2010). Total flavonoids content and biochemical screening of the leaves of tropical endemic medicinal plant Merremia borneensis. Arabian Journal of Chemistry 7:1034-1038. 
Crossref

 

Shobo AO, Anokwuru C, Jokotagba OA, Koleoso OK, Tijani RO (2015). Chemical composition of methanolic extracts of Ocimum canum Sims (Lamiaceae) leaves. International Journal of Medical and Applied Sciences 4:118-123.

 

Siti HN, Kamisaha Y, Kamsiaha J (2015). The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease (a review). Vascular Pharmacology 71:40-56. 
Crossref

 

Srinivas SR (2015). Ethno botanical study of medicinal plants of Sri Pancha Narasimha Swamy and Sri Matsyagiri Narasimha Swamy. Journal of Medicinal Plants Studies 3(3):37-42.

 

Sung JH, Jo YS, Kim SJ, Ryu JS, Kim MC, Ko HJ, Sim SS (2013). Effect of lutein on L-NAME-induced hypertensive rats. The Korean Journal of Physiology and Pharmacology 17(4):339-345.
Crossref

 

Talbi H, Boumaza A, El-mostafa K, Talbi J, Hilali A (2015). Evaluation de l'activité antioxydante et la composition physico-chimique des extraits méthanolique et aqueux de la Nigella sativa L. (Evaluation of antioxidant activity and physico-chemical composition of methanolic and aqueous extracts of Nigella sativa L.). Journal of Material and Environnemental Science 6(4):1111-1117

 

Tarnaud L, Garcia C, Krief S, Simmen B (2010). Apports nutritionnels, dépense et bilan énergétiques chez l'homme et les primates non-humains: aspects méthodologiques. Revues De Primatologie 2:558.
Crossref

 

Tsobou R, Mapongmetsem PM, Voukeng KI, Van Damme P (2015). Phytochemical screening and antibacterial activity of medicinal plants used to treat typhoid fever in Bamboutos division, West Cameroon. Journal of Applied Pharmaceutical Science 5(6):34-49. 
Crossref

 

Turgut DC, Saydam F, Özbayer C, DoÄŸaner F, Soyocak A, GüneÅŸ HV, DeÄŸirmenci Ä°, Kurt H, Üstüner MC, Bal C. (2015). Impact of tannic acid on blood pressure, oxidative stress and urinary parameters in L-NNA-induced hypertensive rats. Cytotechnology 67(1):97-105. 
Crossref

 

Udegbunam SO, Udegbunam RI, Chijioke CM, Madubuike UA, Nwaehujor CO (2014). Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats. BMC Complementary and Alternative Medicine 14:157. 
Crossref

 

Ugusman A, Zakaria Z, Chua KH, Nordin NA, Abdullah Mahdy Z (2014). Role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells. The Scientific World Journal 2014, ID 169370, 9 pages 
Crossref

 

Veerappan R, Senthilkumar R (2015). Chrysin enhances antioxidants and oxidative stress in L-NAME-induced hypertensive rats. International Journal of Nutrition, Pharmacology, Neurological Diseases 5(1):20-27. 
Crossref

 

Veerappan R, Thekkumalai M (2018). Chrysin Pretreatment Improves Angiotensin System, cGMP Concentration in L-NAME Induced Hypertensive Rats. Indian Journal of Clinical Biochemistry. 
Crossref

 

Wagner H, Bladt S (2001). Plant Drug Analysis: A Thin Layer Chromatography Atlas. ISBN 3-540-58676-8 2nd éd. Springer-Verlag New York pp. 359-364.

 

World Health Organization (WHO) (2013). A global brief on Hypertension: Silent killer, global public health crisis. WHO_DCO_WHD_2013.2. P 40
Crossref

 

World Health Organization (WHO) (2009). Report of the WHO Inter-regional Workshop on the Use of Traditional Medicine in Primary Health Care. ISBN 978 92 4 159742.

 

Zicha J, Dobesova Z and Kunes J (2006). Antihypertensive Mechanisms of Chronic Captopril or N-Acetylcysteine Treatment in L-NAME Hypertensive Rats. Hypertension Research 29(12):1021-1027. 
Crossref