Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 415

Full Length Research Paper

Interaction in the production of biofilm and drug susceptibility of Candida kefyr with Escherichia coli and Streptococcus dysgalactiae isolated from bovine mastitis

Israel Daniel Ricardo-González
  • Israel Daniel Ricardo-González
  • Facultad de MedicinaVeterinaria y Zootecnia, Universidad NacionalAutónoma de Mexico, Av. Universidad 3000 Edificio A, DelegaciónCoyoacán. Col. Cd Universitaria CP 04510 México.
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Laura Hernández-Andrade
  • Laura Hernández-Andrade
  • InstitutoNacional de Investigaciones Forestales, Agrícolas y Pecuarias, CENID-Salud Animal e Inocuidad, INIFAP, Carretera México-Toluca, Km 15.5. Col. Palo Alto, CP 05110. DelegaciónCuajimalpa, Ciudad de México.
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Ana Lilia Del Monte-Rodríguez
  • Ana Lilia Del Monte-Rodríguez
  • InstitutoNacional de Investigaciones Forestales, Agrícolas y Pecuarias, CENID-Salud Animal e Inocuidad, INIFAP, Carretera México-Toluca, Km 15.5. Col. Palo Alto, CP 05110. DelegaciónCuajimalpa, Ciudad de México.
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Marco Antonio Santillán-Flores
  • Marco Antonio Santillán-Flores
  • InstitutoNacional de Investigaciones Forestales, Agrícolas y Pecuarias, CENID-Salud Animal e Inocuidad, INIFAP, Carretera México-Toluca, Km 15.5. Col. Palo Alto, CP 05110. DelegaciónCuajimalpa, Ciudad de México.
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Miguel Ángel Blanco Ochoa
  • Miguel Ángel Blanco Ochoa
  • Facultad de MedicinaVeterinaria y Zootecnia, Universidad NacionalAutónoma de Mexico, Av. Universidad 3000 Edificio A, DelegaciónCoyoacán. Col. Cd Universitaria CP 04510 México.
  • Google Scholar
Luis Octavio Campuzano-Reyes
  • Luis Octavio Campuzano-Reyes
  • Facultad de MedicinaVeterinaria y Zootecnia, Universidad NacionalAutónoma de Mexico, Av. Universidad 3000 Edificio A, DelegaciónCoyoacán. Col. Cd Universitaria CP 04510 México.
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Alberto Jiménez-Saavedra
  • Alberto Jiménez-Saavedra
  • Instituto Mexicanodel Seguro Social, México.
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  •  Received: 18 February 2022
  •  Accepted: 04 July 2022
  •  Published: 31 August 2022

Abstract

Bovine mastitis is a disease with a high economic impact on dairy farms and it has been described that different species of Candida can cause it. The objectives of this study were to evidence the production of biofilm by strains of Candida Kefyr, to carry out a comparative evaluation of its production by tube and plate techniques, to determine its interaction with Escherichia coli and Streptococcus dysgalactiae isolated from bovine mastitis and know the drug susceptibility of each of them. The identification of C. kefyr was carried out by fermentation and assimilation of carbohydrates, the confirmation of the identification and minimum inhibitory concentration to antimycotics was carried out with Vitek 2 Systems. Plate and tube biofilm formation assays were performed in triplicate. Analysis of variance (ANOVA) and Tukey's test were performed, as well as Spearman's correlation to the data obtained. In plate tests, biofilm formation was demonstrated in seven C. kefyr isolates, as well as synergy in combinations with E. coli and S. dysgalactiae. However, the inoculum containing the three microorganisms behaved similarly to that containing only C. kefyr and S. dysgalactiae. It was possible to demonstrate a correlation between biofilm formation tests in tube and plate. This is the first report in Mexico of C. kefyr, as well as the production of biofilm in bovine clinical mastitis.

Key words: Mastitis, Candida kefyr, Escherichia coli, Streptococcus dysgalactiae, biofilm, drug susceptibility