Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 416

Full Length Research Paper

In vitro anthelmintic activity of Leonotis nepetifolia ethanolic and aqueous leaf extracts on Fasciola gigantica

Francis Ssenkuba
  • Francis Ssenkuba
  • Department of Biology, Mbarara University of Science and Technology, Uganda.
  • Google Scholar
Cleophas Komujuni
  • Cleophas Komujuni
  • Department of Chemistry, Mbarara University of Science and Technology, Uganda.
  • Google Scholar
Julius Tumusiime
  • Julius Tumusiime
  • Department of Animal Ecology and Systematics, Justus Liebig University, Giessen, Uganda.
  • Google Scholar


  •  Received: 26 September 2022
  •  Accepted: 02 December 2022
  •  Published: 31 December 2022

References

Ahmad I, Aqil F, Owais M (2006). Medicinal Plant Biotechnology Medicinal Chemistry of Bioactive Natural Products. Provide Valid online source/link

 

Alvarez-Sanchez MA, Mainar-Jaime RC, Perez-Garcia J, Rojo-Vázquez FA (2006). Short Communications Resistance of Fasciola hepatica to triclabendazole and albendazole in sheep in Spain. Veterinary Record 159(13):424.
Crossref

 
 

Anuracpreeda P, Chawengkirttikul R, Ngamniyom A, Panyarachun B, Puttarak P, Koedrith P, Intaratat N (2017). The in vitro anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica. Parasitology 144(14):1931.
Crossref

 
 

Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Jahurul MHA, Ghafoor K, Norulaini NAN, Omar AKM (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering 117(4):426-436.
Crossref

 
 

Balamurugan V, Fatima S, Velurajan S (2019). a Guide To Phytochemical Analysis. International Journal of Advance Research and Innovative Ideas In Education 5(1):236-245.

View

 
 

Bate-Smith EC (1962). The phenolic constituents of plants and their taxonomic significance. I. Dicotyledons. Botanical Journal of the Linnean Society 58(371):95-173.
Crossref

 
 

Boadu AA, Asase A (2017). Documentation of herbal medicines used for the treatment and management of human diseases by some communities in southern Ghana. Evidence-Based Complementary and Alternative Medicine, 2017.
Crossref

 
 

Borgsteede FHM, MollL, Vellema P (2005). Lack of reversion in triclabendazole resistant Fasciola hepatica. Veterinary Record 156(11):350-351.
Crossref

 
 

Bwalya AG (2015). Evaluation of the in vitro biological activities and phytochemical profiling of eight ficus species collected in Zambia. UCL (University College London).

 
 

Cabardo Jr DE, Portugaliza HP (2017). Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae. International Journal of Veterinary Science and Medicine 5(1):30-34.
Crossref

 
 

Castro-Hermida JA, González-WarletaM, Martínez-Sernández V, Ubeira FM, Mezo M (2021). Current challenges for fasciolicide treatment in ruminant livestock. Trends in Parasitology 37(5):430-444.
Crossref

 
 

Chang ACG, Flores MJC (2015). Morphology and viability of adult Fasciola gigantica (giant liver flukes) from Philippine carabaos (Bubalus bubalis) upon in vitro exposure to lead. Asian Pacific Journal of Tropical Biomedicine 5(6):493-496.
Crossref

 
 

Coles GC, Stafford KA (2001). Activity of oxyclozanide, nitroxynil, clorsulon and albendazole against adult triclabendazole-resistant Fasciola hepatica. The Veterinary Record 148(23):723-724.
Crossref

 
 

Dash PR, Mou KM, Erina IN, Ripa FA, Al Masud KN, Ali MS (2017). Study of anthelmintic and insecticidal activities of different extracts of Kaempferia galanga. International Journal of Pharmaceutical Sciences and Research 8(2):729.

 
 

dos Santos Nascimento LB, de Aguiar PF, Leal?Costa MV, Coutinho MAS, Borsodi MPG, Rossi?Bergmann B, Tavares ES, Costa SS (2018). Optimization of aqueous extraction from Kalanchoe pinnata leaves to obtain the highest content of an anti?inflammatory flavonoid using a response surface model. Phytochemical Analysis 29(3):308-315.
Crossref

 
 

Eguale T, Tilahun G, Debella A, Feleke A, Makonnen E (2007). In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus. Journal of Ethnopharmacology 110(3):428-433. 
Crossref

 
 

Fairweather I, Brennan GP, Hanna REB, Robinson MW, Skuce PJ (2020). Drug resistance in liver flukes. International Journal for Parasitology: Drugs and Drug Resistance 12:39-59.
Crossref

 
 

Giang NTH, Van Ngot P, Thanh DTN (2021). Morphological, anatomical and antibacterial characteristics of Leonotis nepetifolia plants growing in Binh Thuan Province, Vietnam. GSC Biological and Pharmaceutical Sciences 14(2):53-63.
Crossref

 
 

Goel V, Kaur P, Singla LD and Choudhury D (2020a). Biomedical evaluation of Lansium parasiticum extract protected silver nanoparticles against Haemonchus contortus, a parasitic worm. Frontiers in Molecular Biosciences 7:595646. 
Crossref

 
 

Goel V, Singla LD and Choudhury D (2020b).Cuminaldehyde induces oxidative stress-mediated physical damage and death of Haemonchus contortus. Biomedicine and Pharmacotherapy 130:110411. 
Crossref

 
 

Goswami S, Pandey A, Tripathi P, Singh A, Rai A (2011). An in vitro evaluation of the anthelmintic activity of Hedychium spichatum rhizomes and Zingiber zerumbet rhizomes on the Pheritima Posthuma model: A comparative study. Pharmacognosy Research 3(2):140.
Crossref

 
 

Gumisiriza H, Sesaazi CD, Olet EA, Kembabazi O, Birungi G (2021). Medicinal plants used to treat" African" diseases by the local communities of Bwambara sub-county in Rukungiri District, Western Uganda. Journal of Ethnopharmacology 268:113-578.
Crossref

 
 

Hansen TVA, Fryganas C, Acevedo N, Caraballo L, Thamsborg SM, Mueller-Harvey I, Williams AR (2016). Proanthocyanidins inhibit Ascaris suum glutathione-S-transferase activity and increase susceptibility of larvae to levamisole in vitro. Parasitology International 65(4):336-339.
Crossref

 
 

Harbonne JB (1993). New naturally occurring plant polyphenols.

 
 

Hiai S, Oura H (1976). Color Reaction of Some Sapogenins With Vanillin Sulphuric Acid. Planta Medica 29(2):116-122.
Crossref

 
 

Hoste H, Karla M (2011). Ultrastructural changes in the third- stage , infective larvae of ruminant nematodes treated with sainfoin.
Crossref

 
 

Hoste H, Quijada J (2016). Interactions Between Nutrition and Infections With Haemonchus contortus and Related Gastrointestinal Nematodes in Small Ruminants. In Advances in Parasitology (Vol. 93). Elsevier Ltd. 
Crossref

 
 

Ibekwe HA (2019). In vitro anthelmintic activities of aqueous crude extract of Azadirachta indica on Paramphistomum cervi and Fasciola hepatica. International Journal of Veterinary Sciences and Animal Husbandry 4(1):14-18.

 
 

Imran S, Suradkar SS, Koche DK (2012). Phytochemical analysis of leonotis nepetifolia (l.) r. br., a wild medicinal plant of lamiaceae. Bioscience Discovery 3(2):196-197.

 
 

Joan N, Stephen MJ, Bashir M, Kiguli J, Orikiriza P, Bazira J, Itabangi H, Stanley IJ (2015a). Prevalence and economic impact of bovine fasciolosis at Kampala City abattoir, Central Uganda.
Crossref

 
 

Joan N, Stephen MJ, Bashir M, Kiguli J, Orikiriza P, Bazira J, Itabangi H, Stanley IJ (2015b). Prevalence and Economic Impact of Bovine Fasciolosis at Kampala City Abattoir , Central Uganda. 7(November 2014):109-117. 
Crossref

 
 

Katoch R (2011). Analytical techniques in biochemistry and molecular biology. Springer Science and Business Media
Crossref

 
 

Kaufman PB., Cseke LJ, Warber S, Duke JA, Brielmann, HL.(1998). Natural products from plants. CRC Press Inc.
Crossref

 
 

Kaur P, Singla LD, Singh E, Pawar P (2015) Anthelmintic resistance menace in small ruminants: Solutions to this emerging issue. Punjab Veterinary Journal (June-December) 81-85.

 
 

Kelley JM, Elliott TP, Beddoe T, Anderson G, Skuce P, Spithill TW (2016). Current threat of triclabendazole resistance in Fasciola hepatica. Trends in Parasitology 32(6):458-469.
Crossref

 
 

Khan AM, Bhadauria S, Yadav R (2019). Phytochemical Screening and Antioxidant activity of extract of different parts of Adhatoda vasica. Research Journal of Pharmacy and Technology, 12(12):5699-5705.
Crossref

 
 

Klongsiriwet C, Quijada J, Williams AR, Mueller-Harvey I, Williamson EM, Hoste H (2015). Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. International Journal for Parasitology: Drugs and Drug Resistance 5(3):127-134.
Crossref

 
 

Mahardika MM, Sudjarwo SA, Koesdarto S (2017). Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro. KnE Life Sciences, 266-277.
Crossref

 
 

Malatji MP, Pfukenyi DM, Mukaratirwa S (2020). Fasciola species and their vertebrate and snail intermediate hosts in East and Southern Africa: a review. Journal of Helminthology, p. 94.
Crossref

 
 

Manke MB, Dhawale SC, Jamkhande PG (2015). Helminthiasis and medicinal plants: a review. Asian Pacific Journal of Tropical Disease 5(3):175-180.
Crossref

 
 

Martin RJ (1997). Modes of action of anthelmintic drugs. The Veterinary Journal 154(1):11-34.
Crossref

 
 

Mata-Santos T, Mata-Santos HA, Carneiro PF, De Moura KCG, Fenalti JM, Klafke GB, Cruz LAX, Martins LHR, Pinto NF, Pinto M (2016). Toxocara canis: anthelmintic activity of quinone derivatives in murine toxocarosis. Parasitology 143(4):507-517.
Crossref

 
 

Mbanzamihigo L, Dethie F, Kabera NJ, Ugirinshuti V, Nyirabageni A (2013). Evaluation of the effectiveness of two medicinal plants Vernonia amygdalina and Leonotis nepetaefolia on the gastrointestinal parasites of goats in Rwanda: Case study of Huye and Gisagara districts. Journal of Veterinary Medicine and Animal Health 5(8):229-236.

 
 

Nasma A, Aishath N, Azilah A, Sulaiman AZ (2018). Optimization of vitexin and isovitexin compounds extracted from dried Mas Cotek leaves using one-factor-at-a-time (OFAT) approach in aqueous extraction. International Food Research Journal 25(6):2560-2571.

 
 

Ndjonka D, Abladam ED, Djafsia B, Ajonina-Ekoti I, Achukwi MD, Liebau E (2014). Anthelmintic activity of phenolic acids from the axlewood tree Anogeissus leiocarpus on the filarial nematode Onchocerca ochengi and drug-resistant strains of the free-living nematode Caenorhabditis elegans. Journal of Helminthology 88(4):481-488. 
Crossref

 
 

Ndjonka D, Agyare C, Lüersen K, Djafsia B, Achukwi D, Nukenine EN, Hensel A, Liebau E (2011). In vitro activity of Cameroonian and Ghanaian medicinal plants on parasitic (Onchocerca ochengi) and free-living (Caenorhabditis elegans) nematodes. Journal of Helminthology 85(3):304-312. 
Crossref

 
 

Olaechea F (2004). Red de Helmintología de FAO para América Latina y el Caribe. Conferencia Electrónica.

 
 

World Health Organization (WHO) (2010). Working to overcome the global impact of neglected tropical diseases: first WHO report on neglected tropical diseases (Issue WHO/HTM/NTD/2010.1).

 
 

World Health Organization (WHO) (2013). WHO traditional medicine strategy: 2014-2023.

 
 

Pawar PD, Singla LD, Kaur P Bal MS and Javed M (2019). Evaluation and correlation of multiple anthelmintic resistance to gastrointestinal nematodes using different faecal egg count reduction methods in small ruminants of Punjab, India. Acta Parasitologica 64:456-463.
Crossref

 
 

Perrett S, Whitfield PJ (1995). Atanine (3-dimethylallyl-4-methoxy-2- quinolone), an alkaloid with anthelmintic activity from the Chinese medicinal plant, Evodia rutaecarpa. Planta Medica 61(03):276-278.
Crossref

 
 

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin JC, Bailleul F, Trotin F (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology 72(1-2):35-42.
Crossref

 
 

Rehbein S, Visser M (1999). Efficacy of an injectable ivermectin/clorsulon combination against Fasciola hepatica in sheep. Veterinary Record 145(16):468.
Crossref

 
 

Roy H, Chakraborty A, Bhanja S, Nayak BS, Mishra SR, Ellaiah P (2010). Preliminary phytochemical investigation and anthelmintic activity of Acanthospermum hispidum DC. Journal of Pharmaceutical Science and Technology 2(5):217-221.

 
 

Samje M, Metuge J, Mbah J, Nguesson B, Cho-Ngwa F (2014). In vitro anti-Onchocerca ochengi activities of extracts and chromatographic fractions of Craterispermum laurinum and Morinda lucida. BMC Complementary and Alternative Medicine 14(1):1-12. 
Crossref

 
 

Santos ACV, Santos FO, Lima HG, Da Silva GD, Uzêda RS, Dias ÊR, Branco A, Cardoso KV, David JM, Botura MB (2018). In vitro ovicidal and larvicidal activities of some saponins and flavonoids against parasitic nematodes of goats. Parasitology 145(14):1884-1889.
Crossref

 
 

Santos FO, Cerqueira APM, Branco A, Batatinha MJM, Botura MB (2019). Anthelmintic activity of plants against gastrointestinal nematodes of goats: A review. Parasitology, 146(10):1233-1246.
Crossref

 
 

Shokier KM, Aboelhadid SM, Waleed MA (2013). Efficacy of five anthelmintics against a natural Fasciola species infection in cattle. Beni-Suef University. Journal of Basic and Applied Sciences 2(1):41-45.
Crossref

 
 

Simoben CV, Ntie-Kang F, Akone SH, Sippl W (2018). Compounds from African Medicinal Plants with Activities against Selected Parasitic Diseases: Schistosomiasis, Trypanosomiasis and Leishmaniasis. Natural Products and Bioprospecting 8(3):151-169. 
Crossref

 
 

Soldera-Silva A, Seyfried M, Campestrini LH, Zawadzki-Baggio SF, Minho AP, Molento MB, Maurer JBB (2018). Assessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts. Veterinary Parasitology 251:34-43.
Crossref

 
 

Stepek G, Lowe AE, Buttle DJ, Duce IR, Behnke JM (2006). In vitro and in vivo anthelmintic efficacy of plant cysteine proteinases against the rodent gastrointestinal nematode, Trichuris muris. Parasitology 132(5):681-689.
Crossref

 
 

Sumbria D, Singla LD (2020). Fascioliasis in ruminants. Livestock line 13(12):10-11.

 
 

Tariq KA, Chishti MZ, Ahmad F, Shawl AS (2009). Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes. Veterinary Parasitology 160(1-2):83-88. 
Crossref

 
 

Tarnopolsky MA, Beal MF (2001). Potential for creatine and other therapies targeting cellular energy dysfunction in neurological disorders. Annals of Neurology 49(5):561-574.
Crossref

 
 

Vasuki K, Murugananthan G, Ragavi P (2017). Phytochemical and Anthelmintic Studies on the Whole Plant of Leonotis Nepetifolia (L). R. BR.

 
 

Walker SM, Makundi AE, Namuba FV, Kassuku AA, Keyyu J, Hoey EM, Prödohl P, Stothard JR, Trudgett A (2008). The distribution of Fasciola hepatica and Fasciola gigantica within southern Tanzania-constraints associated with the intermediate host. Parasitology 135(4):495-503. 
Crossref

 
 

Waller PJ (1997). Sustainable helminth control of ruminants in developing countries. Veterinary Parasitology 71(2-3):195-207.
Crossref

 
 

Wangchuk P, Giacomin PR, Pearson MS, Smout MJ, Loukas A (2016). Identification of lead chemotherapeutic agents from medicinal plants against blood flukes and whipworms. Scientific Reports 30;6(1):1-0.
Crossref

 
 

Williams AR, Fryganas C, Ramsay A, Mueller-harvey I, Thamsborg SM (2014). Direct anthelmintic effects of condensed tannins from diverse plant sources against Ascaris suum. PloS one 9(5):e97053.
Crossref

 
 

Wynn SG, Fougère B (2006). Veterinary herbal medicine. Elsevier Health Sciences P 695.