Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2744

Full Length Research Paper

Phenotypic screening of multidrug-resistant E. coli from water and fish collected from different fish farms within Abakaliki metropolis, Nigeria

Chibuike K. U.
  • Chibuike K. U.
  • Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P. M. B. 053, Ebonyi State, Nigeria.
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Iroha I. R.
  • Iroha I. R.
  • Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P. M. B. 053, Ebonyi State, Nigeria.
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Moses I. B.
  • Moses I. B.
  • Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P. M. B. 053, Ebonyi State, Nigeria.
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Chukwunwejim C. R.
  • Chukwunwejim C. R.
  • Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.
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Peter I. U.
  • Peter I. U.
  • Department of Public Health, Faculty of Health Technology and Engineering, Federal College of Dental Technology and Therapy, Trans-Ekulu, Enugu State, Nigeria.
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Edemekong C. I.
  • Edemekong C. I.
  • Department of Biotechnology, Faculty of Applied Sciences, Federal College of Dental Technology and Therapy, Trans-Ekulu, Enugu State, Nigeria.
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Ndugo C.M.
  • Ndugo C.M.
  • Medical Laboratory Department, St. Vincent Hospital, Ndubia Igbeagu, Izzi LGA, Ebonyi State.
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Ngene O.
  • Ngene O.
  • Medical Laboratory Department, University of Nigeria Teaching Hospital, Ituku Ozala, Enugu State.
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Egbuna N. R.
  • Egbuna N. R.
  • Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Chukwuma Odumegwu Ojukwu University, Igabaram, Anambra State, Nigeria.
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Okonkwo-Uzor N. J.
  • Okonkwo-Uzor N. J.
  • Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Chukwuma Odumegwu Ojukwu University, Igabaram, Anambra State, Nigeria.
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  •  Received: 04 November 2020
  •  Accepted: 17 December 2020
  •  Published: 30 June 2021

Abstract

The aim of this study was to screen fish farm water and fishes reared in fish farms within Abakaliki metropolis for multi-drug resistant Escherichia coli. Exactly 30 fish samples and 30 water samples were obtained from 10 different fish farms using sterile bottles from January to June, 2018. Samples were analyzed by standard microbiology methods. Susceptibility test to antibiotics was done by Kirby-Bauer disc diffusion technique. Double-disc synergy test was used to screen isolates for Extended Spectrum Beta-Lactamase (ESBL)-production. Exactly 54 (60%) E. coli and 12 (40%) E. coli were recovered from 90 different fish parts and 30 farm water samples, respectively. Isolates exhibited resistance (54% - 100%) to ceftazidime, aztreonam, cefotetan, cefuroxime, cefoxitin, piperacillin/tazobactam, ofloxacin, and ceftriaxone, but were susceptible to imipenem (80%), cefotaxime (60%), and gentamicin (57%). All the E. coli isolates form water and fish samples were negative for ESBL production.  An average multiple antibiotic resistance index (MARI) value of 0.71 was recorded for the isolates. High prevalence of E. coli with multidrug-resistant traits in fish and water samples in our study area is a serious public health concern as this will make the treatment of infections, especially E. coli-associated foodborne diseases very difficult, thus leading to increase in health care cost, morbidity, and mortality.

Key words: Escherichia coli, fish farm, multidrug-resistance, farm water, fish.