Abstract

We have investigated the feasibility of an approach that combines a non-PCR-based amplification of the target DNA to a microarray-based detection of the single nucleotide polymorphisms (SNPs) in genes associated with bacterial resistance to beta-lactams. The method involves a random non-PCR amplification by the exo-Klenow, followed by hybridization on a low-density DNA chip. This approach was demonstrated to produce specific hybridization using recombinant and natural plasmids harboring bla_SHV variants as model of SNP-containing genes.

Key words: Antibiotic resistance, non-PCR amplification, hybridization, DNA chip, mutation.