The immature embryos of Clivia miniata Regel. cv. Ranchang were in vitro induced with 0.00, 0.01, 0.03 and 0.05% colchicine treatment for 10, 20 and 30 d, respectively on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 a-Naphthaleneacetic acid (NAA) and 1.5 mg L-1 6-Benzylaminopurine (BA). A total of 14 tetraploids were obtained with an average induction rate of 16.1%. The most effective treatment was 0.03% colchicine for 20 days with an induction rate of 30.0%. The stomata size (37.24 × 12.31 μm) of tetraploids was significantly larger than that of diploids (22.51 × 7.43 μm). The stomata density of tetraploids (13.80 mm2) was significantly lower than that of diploids (30.78 mm2). The chloroplast number of guard cells of tetraploids (41.53) was significantly more than that of diploids (28.00). Compared with diploids, tetraploids had thicker, wider, shorter, rougher and deeper-colorful leaves, fewer roots and slower growth. The leaf and stoma characteristics could be regarded as helpful indexes to identify colchicine-induced tetraploids in C. miniata.
Key words: Clivia miniata Regel., chromosome doubling, colchicine, tetraploid, stoma, chloroplast number.
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