African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6934

Full Length Research Paper

Detection of Staphylococcus species and staphylococcal enterotoxins by ELISA in ice cream and cheese consumed in Burdur Province

Fulya Tasci1*, Fatma Sahindokuyucu2 and Dilek Ozturk3
  1Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15100, Burdur, Turkey. 2Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15100, Burdur, Turkey. 3Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15100, Burdur, Turkey.
Email: [email protected]

  •  Accepted: 12 January 2011
  •  Published: 18 February 2011

Abstract

 

In this study, 50 ice cream and 50 cheese samples from Burdur Province of Turkey were examined for total mesophilic aerobic bacteria, Micrococcus/Staphylococcus and Staphylococcus aureus. In ice cream, total aerobic mesophilic microorganisms, Micrococcus/Staphylococcus and S. aureus were determined at levels of 4.29, 3.78 and 3.00 log10cfu/g, respectively. In cheese, these organisms were present at 8.21, 6.21 and 5.80 log10cfu/g, respectively. Enterotoxin analyses were performed with Enzyme Linked Immunosorbent Assay (ELISA). Enterotoxins were detected in 3 cheese samples and were identified as staphylococcal enterotoxins B, C and E. Further attention should be given to the bacteriological standards for milk used in cheese and ice cream production.

 

Key words: Staphylococcal enterotoxin, ELISA, cheese, ice cream.

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