African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6842

Full Length Research Paper

Isolation and selection of P-solubilizing and IAA-synthesizing microorganisms from the rhizosphere of Guanandi (Calophyllum brasiliensis)

Luiz Carlos Ramos dos Santos
  • Luiz Carlos Ramos dos Santos
  • Instituto Federal Goiano, Campus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
  • Google Scholar
Isabel Cristina Mendonça Cardoso Jakoby
  • Isabel Cristina Mendonça Cardoso Jakoby
  • Instituto Federal Goiano, Campus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
  • Google Scholar
Marcos Antônio Soares
  • Marcos Antônio Soares
  • Laboratório de Biotecnologia e Ecologia Microbiana, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa, 2367, Boa Esperança 78060-900, Cuiabá, Mato Grosso, Brasil.
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Edson Luiz Souchie
  • Edson Luiz Souchie
  • Instituto Federal Goiano, Campus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
  • Google Scholar
Alan Carlos Costa
  • Alan Carlos Costa
  • Instituto Federal Goiano, Campus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil
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  •  Received: 19 February 2015
  •  Accepted: 18 September 2015
  •  Published: 03 December 2015

Abstract

Phosphate solubilization by microorganisms is an important process for increasing the bioavailability of phosphorus (P) to plants. This study aimed to: (a) in solid medium, isolate CaHPO4-solubilizing and non-P-solubilizing microorganisms from the rhizosphere of guanandi (Calophyllum brasiliensis) collected from a nursery and the field; (b) in liquid media, quantify the ability of CaHPO4-solubilizing isolates from the rhizosphere of guanandi to solubilize FePO4; and (c) quantify indoleacetic acid (IAA) production by FePO4-solubilizing rhizosphere microorganisms. Two guanandi plants, one cultivated in nursery and another from the field, were used for the isolation of FePO4 and CaHPO4-solubilizing bacteria and fungi. The largest population of P-solubilizing and non-P-solubilizing microorganisms was found in the rhizosphere of guanandi cuttings cultivated in the field. The abilities to solubilize FePO4 and produce IAA are variable among guanandi rhizosphere isolates, where bacterial isolates produce higher levels of IAA than fungal isolates.

 

Key words: Phosphorus, auxin, phosphate solubilization, seedlings.