African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

Antimicrobial action against of Macrophomina phaseolina and control of the grey stem in soybean by homeopathic remedies Nosode and Sulphur

Eloisa Lorenzetti
  • Eloisa Lorenzetti
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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José Renato Stangarlin
  • José Renato Stangarlin
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Elizana Lorenzetti Treib
  • Elizana Lorenzetti Treib
  • Campo Comprido, Positive University, UP, Curitiba Street, Professor Pedro Viriato Parigot de Souza, Number 5300, Zip Code: 81280-330. Paraná Brazil.
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Anderson Luis Heling
  • Anderson Luis Heling
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Sidiane Coltro-Roncato
  • Sidiane Coltro-Roncato
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Jeferson Carlos Carvalho
  • Jeferson Carlos Carvalho
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Lívia Hoepers
  • Lívia Hoepers
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Bruna Broti Rissato
  • Bruna Broti Rissato
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Jéssica Caroline Coppo
  • Jéssica Caroline Coppo
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Cristiani Belmonte
  • Cristiani Belmonte
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Odair José Kuhn
  • Odair José Kuhn
  • Marechal Cândido Rondon, State University of West Paraná, UNIOESTE, Street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Paraná, Brazil.
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Itamar Ferreira da Silva
  • Itamar Ferreira da Silva
  • Toledo Paraná. Avenue União, Pontifical Catholic University of Paraná, PUCPR, Number 500, Zip Code: 85902-532, Brazil.
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  •  Received: 16 May 2016
  •  Accepted: 22 August 2016
  •  Published: 08 September 2016

Abstract

The aims of the study were to evaluate the control of botrytis in the soybean stem, spraying homeopathic solutions of Sulphur and Nosode of Macrophomina phaseolina in the dynamizations 6, 12, 24, 36 and 48CH. As additional treatment, we used distilled water and hydroalcoholic solution at 30% ethanol. In vitro tests were performed so as to analyze the number of micro-sclerotia and mycelial growth of the fungi, and in vivo tests to track the progress of the botrytis stem and the size of the lesion. The experimental design was in randomized blocks, with five replications. For the area under the mycelial growth curve (AUMGC) in the first test, there was no significant effect of Sulphur and Nosode of M. phaseolina. In vivo studies, comparing Sulphur with the control treatment, showed a reduction of 14 and 15% for the dynamizations of 12 and 48 CH, respectively. For the amount of micro-sclerotia of M. phaseolina, in the first test, Sulphur showed a reduction of up to 50%; Nosode of M. phaseolina showed no significant reduction of micro-sclerotia. In the second test, Nosode was not significant while Sulphur caused a reduction of up to 33%. For area under the disease progress curve (AUDPC), in both trials, the drugs proved to be ineffective. These results indicate the potential of these homeopathic remedies in controlling the fungus, M. phaseolina.

Key words: Alternative control, glycine max, homeopathy.